Anti-atopic dermatitis effects and the mechanism of lactic acid bacteria isolated from Mongolian fermented milk

• Published in: Journal of dairy research Vol. 76; no. 2; pp. 158 - 164
• Main Authors: Hayashi, Atsushi, Kimura, Makoto, Nakamura, Yusaku, Yasui, Hisako
• Format: Journal Article
• Language: English
• Published: Cambridge, UK Cambridge University Press 01.05.2009
• Subjects: Allergies; animal models; Animal productions; Animals; anti-allergy; anti-allergy properties; antigen-presenting cells; atopic dermatitis; Bacteria; Biological and medical sciences; Cells, Cultured; Cultured Milk Products - microbiology; Cytokines - metabolism; dendritic cells; Dermatitis; Dermatitis, Atopic - prevention & control; Enterococcus faecium; Enterococcus faecium - physiology; fermented milk; Food industries; Food microbiology; Fundamental and applied biological sciences. Psychology; human diseases; hypersensitivity; immunoglobulin E; interferons; interleukin-12; Lactic Acid - metabolism; lactic acid bacteria; macrophages; Male; medicinal properties; Mice; Mice, Inbred Strains; Mongolia; Monoclonal antibodies; NC/Nga mouse; Neutralization; Peyer's Patches - cytology; Peyer's Patches - metabolism; Probiotics; Rodents; Skin diseases; Small intestine; Specific Pathogen-Free Organisms; Spleen - cytology; Spleen - metabolism; splenocytes; Studies; Terrestrial animal productions; Vertebrates; Mongolia; atopic dermatitis; lactic acid bacteria; NC/Nga mouse; anti-allergy; Immunopathology; Allergy; Lactic acid bacteria; Rodentia; Dairy product; Dermatitis; Mechanism; Vertebrata; Mammalia; Mouse; Dairy industry; Cultured milk
• ISSN: 0022-0299; 1469-7629
• DOI: 10.1017/S0022029908003725

We investigated the anti-allergic effects of one strain (T120) of a lactic acid bacteria (LAB) isolated from Mongolian fermented milk using atopic dermatitis (AD) model mice (NC/Nga mice). Strain T120 has already been identified as Enterococcus faecium and shown to induce strong production of IL-12 (Kimura et al. 2006). In in vitro studies, strain T120 suppressed total IgE production and induced IL-12 and IFN-γ production by splenocytes of NC/Nga mice. The additional examination of various neutralization antibodies was performed to elucidate in detail the mechanism of depressed IgE production by strain T120. As a result, it became clear that IL-12 induced by strain T120 increased production of IFN-γ and total IgE production was mainly controlled by the IFN-γ. In order to define the cells which produce IL-12 powerfully by this strain, antigen-presenting cells (APCs) such as macrophages and dendritic cells (DCs) were removed from the splenocytes, and the reactivity of these cells to the strain was examined. Induction of IL-12 and IFN-γ by strain T120 became significantly very low by removal of APCs from splenocytes. Therefore, it was clear that strain T120 acted on APCs and induced production of IL-12. Further, this strain enhanced the production of IL-10 by splenocytes. In in vivo studies, intraperitoneal injection of strain T120 inhibited serum IgE elevation and atopic dermatitis symptoms in NC/Nga mice. These results suggest that an anti-allergic effect of strain T120 depends on the increased production of IL-12 by APCs activated by the strain and following the increased production of IFN-γ. Further, activation of regulatory T cells by strain T120 may inhibit atopic desease.