Identification of a Carbohydrate-Based Endothelial Ligand for a Lymphocyte Homing Receptor

• Published in: The Journal of cell biology Vol. 113; no. 5; pp. 1213 - 1221
• Main Authors: Imai, Yasuyuki, Singer, Mark S., Fennie, Christopher, Lasky, Laurence A., Rosen, Steven D.
• Format: Journal Article
• Language: English
• Published: New York, NY Rockefeller University Press 01.06.1991; The Rockefeller University Press
• Subjects: 550201 - Biochemistry- Tracer Techniques; ADHESION; ANIMAL CELLS; ANIMAL TISSUES; ANIMALS; BASIC BIOLOGICAL SCIENCES; BIOCHEMICAL REACTION KINETICS; Biological and medical sciences; BIOLOGICAL MATERIALS; BLOOD; BLOOD CELLS; BODY; BODY FLUIDS; Butchering; Carbohydrate Metabolism; CARBOHYDRATES; Cell Adhesion; Cell Adhesion Molecules - biosynthesis; Cell Adhesion Molecules - isolation & purification; Cell Adhesion Molecules - physiology; Cell structures and functions; CHEMICAL COMPOSITION; CONNECTIVE TISSUE CELLS; ENDOTHELIUM; Endothelium, Vascular - physiology; Female; Fundamental and applied biological sciences. Psychology; GLYCOPROTEINS; Hepatitis E virus; Homing; HYDROGEN COMPOUNDS; ISOTOPE APPLICATIONS; ISOTOPES; KINETICS; Lectins; LEUKOCYTES; LIGANDS; LYMPH NODES; Lymph Nodes - physiology; LYMPHATIC SYSTEM; LYMPHOCYTES; Lymphocytes - immunology; Lymphocytes - physiology; MAMMALS; MATERIALS; MEL-14 antigen; MEMBRANE PROTEINS; MICE; Mice, Inbred ICR; Molecular and cellular biology; MOLECULAR WEIGHT; Molecules; ORGANIC COMPOUNDS; OXYGEN COMPOUNDS; PROTEINS; REACTION KINETICS; RECEPTORS; Receptors, Lymphocyte Homing - physiology; RODENTS; SOMATIC CELLS; SULFATES; Sulfates - metabolism; SULFUR COMPOUNDS; SULFUR ISOTOPES; Sulfur Radioisotopes; TISSUES; TRACER TECHNIQUES; Tritium; TRITIUM COMPOUNDS; VERTEBRATES; Ligand binding; Endothelial cell; Membrane receptor; Identification; Lymphocyte
• ISSN: 0021-9525; 1540-8140
• DOI: 10.1083/jcb.113.5.1213

Lymphocyte attachment to high endothelial venules within lymph nodes is mediated by the peripheral lymph node homing receptor (pnHR), originally defined on mouse lymphocytes by the MEL-14 mAb. The pnHR is a calcium-dependent lectin-like receptor, a member of the LEC-CAM family of adhesion proteins. Here, using a soluble recombinant form of the homing receptor, we have identified an endothelial ligand for the pnHR as an ∼50-kD sulfated, fucosylated, and sialylated glycoprotein, which we designate Sgp50(sulfated glycoprotein of 50 kD). Recombinant receptor binding to this lymph node-specific glycoprotein requires calcium and is inhibitable by specific carbohydrates and by MEL-14 mAb. Sialylation of the component is required for binding. Additionally, the glycoprotein is precipitated by MECA-79, an adhesion-blocking mAb reactive with lymph node HEV. A related glycoprotein of ∼90 kD (designated as Sgp90) is also identified.