Rice Bran Feruloylated Oligosaccharides Activate Dendritic Cells via Toll-Like Receptor 2 and 4 Signaling

• Published in: Molecules (Basel, Switzerland) Vol. 19; no. 4; pp. 5325 - 5347
• Main Authors: Lin, Chi, Chen, Hua, Chen, Yu, Chang, Hung, Lin, Ping, Pan, I-Hong, Chen, Der-Yuan, Chen, Chuan, Lin, Su
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 23.04.2014; MDPI
• Subjects: Acids; Animals; Antigens; B7-1 Antigen - genetics; B7-1 Antigen - immunology; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - metabolism; CD40 Antigens - genetics; CD40 Antigens - immunology; Cell Differentiation - drug effects; Chemokines; Coumaric Acids - chemistry; Cytokines; Dendritic cells; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - metabolism; Drug dosages; Extracellular Signal-Regulated MAP Kinases - genetics; Extracellular Signal-Regulated MAP Kinases - immunology; Female; feruloylated oligosaccharides; Food science; Gene Expression Regulation; Histocompatibility Antigens Class I - genetics; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - immunology; Hospitals; Immunologic Factors - isolation & purification; Immunologic Factors - pharmacology; Immunotherapy; Kinases; Lymphocytes; MAP Kinase Kinase 4 - genetics; MAP Kinase Kinase 4 - metabolism; maturation; Medicine; Mice; Mice, Inbred C3H; Mice, Inbred C57BL; NF-kappa B - genetics; NF-kappa B - metabolism; Oligosaccharides - isolation & purification; Oligosaccharides - pharmacology; Oryza - chemistry; p38 Mitogen-Activated Protein Kinases - genetics; p38 Mitogen-Activated Protein Kinases - metabolism; Proteins; Rice; rice bran; Signal Transduction; Toll-like receptor (TLR); Toll-Like Receptor 2 - genetics; Toll-Like Receptor 2 - immunology; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - immunology; Tumor necrosis factor-TNF; Taiwan
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules19045325

This work presents the effects of feruloylated oligosaccharides (FOs) of rice bran on murine bone marrow-derived dendritic cells (BMDCs) and the potential pathway through which the effects are mediated. We found that FOs induced phenotypic maturation of DCs, as shown by the increased expression of CD40, CD80/CD86 and MHC-I/II molecules. FOs efficiently induced maturation of DCs generated from C3H/HeN or C57BL/6 mice with normal toll-like receptor 4 (TLR-4) or TLR-2 but not DCs from mice with mutated TLR4 or TLR2. The mechanism of action of FOs may be mediated by increased phosphorylation of ERK, p38 and JNK mitogen-activated protein kinase (MAPKs) and increased NF-kB activity, which are important signaling molecules downstream of TLR-4 and TLR-2. These data suggest that FOs induce DCs maturation through TLR-4 and/or TLR-2 and that FOs might have potential efficacy against tumor or virus infection or represent a candidate-adjuvant approach for application in immunotherapy and vaccination.