Gravitropic response of acetylcholinesterase and IAA-inositol synthase in lazy rice [Oryza sativa]

• Published in: Plant production science Vol. 3; no. 1; pp. 17 - 23
• Main Authors: Momonoki, Y.S. (Tokyo Univ. of Agriculture (Japan)), Kawai, N, Takamure, I, Kowalczyk, S
• Format: Journal Article
• Language: English
• Published: Tokyo Taylor & Francis 2000; Crop Science Society of Japan; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: ACETILCOLINESTERASA; ACETYLCHOLINESTERASE; Ageotropic rice; Agronomy. Soil science and plant productions; AIA; Asymmetric distribution; Biological and medical sciences; DISTRIBUCION GEOGRAFICA; DISTRIBUTION GEOGRAPHIQUE; Economic plant physiology; ESTIMULO; Fundamental and applied biological sciences. Psychology; GEOGRAPHICAL DISTRIBUTION; GRAVIDEZ; Gravistimulus; GRAVITE; GRAVITY; IAA; IAA-inositol synthase; INOSITOL; LIGASAS; LIGASE; LIGASES; ORYZA SATIVA; PLANT RESPONSE; REPONSE DE LA PLANTE; RESPUESTA DE LA PLANTA; STIMULI; STIMULUS; Tropism and nastic movements; Indoleacetylglucose-inositol O-acyltransferase; Monocotyledones; Auxin; Neostigmine bromide; Esterases; Colorimetry; Histochemical reaction; Acetylcholinesterase; Immunochemistry; Cereal crop; Oryza sativa; Enzymatic activity; Gramineae; Indoleacetic acid; Angiospermae; Plant growth substance; Geotropism; Acyltransferases; Enzyme; Transferases; Carboxylic ester hydrolases; Hydrolases; Gravitropism; Spermatophyta; Detection; Enzymatic reaction
• ISSN: 1343-943X; 1349-1008
• DOI: 10.1626/pps.3.17

We previously observed that gravistimulation changes the localization of acetylcholinesterase (AChE) and IAA-inositol synthase in maize shoots. In the present study, we analyzed the localization patterns of these enzymes in a lazy strain of rice, which lacks gravitropic responses. AChE was detected by color development due to enzymic reaction in the coleoptile of dark-grown rice plants under a light microscope. IAA-inositol synthase was detected immunochemically using an anti-IAA-inositol synthase polyclonal antibody. Gravistimulation was given by moving 5-d-old rice seedlings from a vertical to a horizontal position. AChE and IAA-inositol synthase in both normal and lazy rice strains were both distributed asymmetrically in the vascular bundles in the lower half of the horizontally oriented coleoptile at the 90th min. At the 4th h, they were distributed asymmetrically in normal rice, but symmetrically in the lazy rice. The immunoreaction of IAA-inositol synthase in the lazy rice was weak compared to that of the normal rice. Neostigmine bromide inhibited AChE activity and asymmetric distribution of IAA-inositol synthase in both normal and lazy rice strains. The results showed that AChE responded to gravistimulus, and consequently controlled the distribution of IAA-inositol synthase in both normal and lazy rice strains.