QTL mapping of antibiosis resistance to common cutworm (Spodoptera litura Fabricius) in soybean

• Published in: Crop science Vol. 45; no. 5; pp. 2044 - 2048
• Main Authors: Komatsu, K, Okuda, S, Takahashi, M, Matsunaga, R, Nakazawa, Y
• Format: Journal Article
• Language: English
• Published: Madison, WI The Crop Science Society of America, Inc 01.09.2005; Crop Science Society of America; American Society of Agronomy
• Subjects: Agricultural management; Agronomy. Soil science and plant productions; alleles; antibiosis; Biological and medical sciences; chromosome mapping; Crop damage; Crop science; Cultivars; Diseases and pests; field crops; Fundamental and applied biological sciences. Psychology; Gene mapping; gene segregation; Genetics and breeding of economic plants; Genomics; Genotypes; Glycine max; homozygosity; insect pests; Pest animals; Pest resistance; plant pests; quantitative trait loci; Soybean; Soybeans; Spodoptera litura; Variance analysis; Varietal selection. Specialized plant breeding, plant breeding aims; Worms; Asia; Japan; Far east; Genetic mapping; Insecta; Antibiosis; Glycine max; Quantitative trait loci; Grain legume; Pest; Spodoptera litura; Leguminosae; Arthropoda; Dicotyledones; Insect resistance; Angiospermae; Lepidoptera; Spermatophyta; Noctuidae; Invertebrata; Cultivar
• ISSN: 0011-183X; 1435-0653
• DOI: 10.2135/cropsci2004.0744

The common cutworm (CCW; Spodoptera litura Fabricius; Lepidoptera: Noctuidae) is a major pest of soybean [Glycine max (L.) Merr.] in Japan. Previously, we analyzed a soybean cultivar named Himeshirazu and found that it exhibits a heritable antibiosis resistance to CCW. In this study, we performed quantitative trait locus (QTL) analysis using composite interval mapping (CIM) method to locate the source of the antibiosis resistance of Himeshirazu. We used a previously reported F2 segregating population of susceptible 'Fukuyutaka' x resistant Himeshirazu. We constructed a linkage map spanning 2270.4 cM with 146 simple sequence repeat (SSR) markers and a phenotype marker (pubescence color). Two QTLs for antibiosis resistance were detected in linkage group (LG) M. One was located between SSR markers Satt220 and Satt175, with a maximum logarithm of odds (LOD) score of 12.7 and r2 = 0.283. The other was located between Satt567 and Satt463, with a maximum LOD score of 6.8 and r2 = 0.159. The resistant allele of both QTLs was derived from Himeshirazu. One-way ANOVA of the F2 family classified by genotypes of SSR loci linked to each QTL suggested that a significant antibiosis effect was exhibited when both QTLs were homozygous for the resistant allele.