Effects of various anti-asthmatic agents on mite allergen-pulsed murine bone marrow-derived dendritic cells

• Published in: Clinical and experimental allergy Vol. 35; no. 7; pp. 884 - 888
• Main Authors: Machida, I., Matsuse, H., Kondo, Y., Kawano, T., Saeki, S., Tomari, S., Obase, Y., Fukushima, C., Kohno, S.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.07.2005; Blackwell; Wiley Subscription Services, Inc
• Subjects: airway inflammation; Albuterol - analogs & derivatives; Albuterol - pharmacology; Allergic diseases; Androstadienes - pharmacology; Animals; Anti-Asthmatic Agents - pharmacology; Antigens, Dermatophagoides - immunology; asthma; Asthma - immunology; Biological and medical sciences; Bone Marrow Cells - drug effects; Bone Marrow Cells - immunology; Bronchoconstriction - immunology; Bronchodilator Agents - pharmacology; Chromones - pharmacology; corticosteroids; cysteinyl leukotriene receptor antagonists; dendritic cells; Dendritic Cells - drug effects; Dendritic Cells - immunology; Female; Fluticasone; Fundamental and applied biological sciences. Psychology; Fundamental immunology; IL-10; IL-12; Immunopathology; Interleukin-10 - immunology; Interleukin-12 - immunology; Medical sciences; Mice; Mice, Inbred BALB C; mite allergen; mouse model; Respiratory System - immunology; Salmeterol Xinafoate; Th2 Cells - immunology; β2-agonists; airway inflammation; Allergy; Animal model; Agonist; β2-agonists; Mite; Respiratory system; Respiratory tract; Immunology; dendritic cells; corticosteroids; Antigen presenting cell; Bone marrow; Bronchus disease; Leukotriene antagonist; Obstructive pulmonary disease; Biological receptor; Immunopathology; Lung disease; Dendritic cell; Corticosteroid; mouse model; Respiratory disease; Rodentia; Cytokine; mite allergen; Interleukin 12; Inflammation; IL-12; IL-10; Asthma; cysteinyl leukotriene receptor antagonists; Vertebrata; Mammalia; Mouse; Animal; Interleukin 10; Allergen
• ISSN: 0954-7894; 1365-2222
• DOI: 10.1111/j.1365-2222.2005.02262.x

Summary Background Dendritic cells (DCs) play an important role in the immune response and are critically involved in asthma. β2‐agonists could potentially exacerbate type 2 T helper (Th2) cell‐mediated immune response. Objectives To determine the effects of various anti‐asthmatic agents on DCs function both in vitro and in vivo. Methods Murine bone marrow‐derived DCs were pulsed with mite allergen in the presence of pranlukast, salbutamol, salmeterol or fluticasone. These DCs were then inoculated intranasally into naïve mice to induce allergic airway inflammation in vivo. Results Pranlukast reduced IL‐10 and increased IL‐12, while fluticasone reduced both IL‐10 and IL‐12 production by mite allergen‐pulsed DCs. Allergic airway inflammation in pranlukast‐ and fluticasone‐treated and mite allergen pulsed DCs‐harbouring mice was attenuated and such response was associated with inhibition of Th2 response in the airway. Salbutamol did not alter cytokine production, while salmeterol reduced IL‐12 production by mite allergen‐pulsed DCs. Lung pathology in β2‐agonist‐harbouring mice was comparable with those of mite allergen‐pulsed DCs‐harbouring mice. Conclusions Our results indicate that leukotriene receptor antagonists and corticosteroids inhibit DCs‐induced Th2 skewed immune response, and that short‐ and long‐acting β2‐agonists do not modify DCs‐induced allergic airway inflammation.