DES Mutagenesis of Antagonistic Streptomyces of Botryosphaeria dothidea and Optimization of Its Fermentation Conditions

• Published in: Plant Diseases and Pests(植物病虫害研究：英文版) Vol. 8; no. 1; pp. 15 - 20
• Main Authors: Limei, Li, Tiantian, Zheng, Yan, Sui, Xin, Li, Jinghua, Sun, Linhao, Zhang, Chunyu, Zhu, Yuequ, Chen
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.02.2017
• Subjects: DES; 产生菌; 发酵条件优化; 拮抗链霉菌; 最佳发酵条件; 最佳培养基; 正交试验设计; 诱变处理
• ISSN: 2152-3932

[ Objective] The paper wag to improve the antimicrobial effect of antagonistic Streptomyces on Botryosphaeria dothidea. [ Method ] Different concentrations of DES were adopted for mutagenic treatment of antagonistic Streptomyces F-58. After fermentation, the mutant strain numbered F-58-06 with genetic stability and good antimicrobial effect was selected among 200 mutant strains; the biological activity of the strain was determined with B. dothidea as the indicator fungus, and the best concentration of DES wag 2%. The fermentation conditions of antagonistic substances of Streptomyces strain F-58-06 produced in shake flasks were opti- mized using the method of single factor and orthogonal experimental design. [ Result] The optimal fermentation conditions were carbon source corn flour, nitrogen source KNO3, initial pH 7, loading volume 30 mL in 250 mL shake flask, inoculation volume 3%, incubation time 132 h. Based on orthogonal experiments, the best cultured medium ratio was corn flour 4%, KNO3 0.5%, CaCO3 0.2%, NaCI 0.35%, MgSO4 · 7H2O 0. 15%. [ Conclusion ] After mutation and optimization of culture medium, the antimicrobial activity of Streptomyces F-58 was greatly improved, and the average inhibition zone diameter was 1.35 times of that of the original strain.