Liposome and QS-21 Combined Adjuvant Induces theHumoral and Cellular Responses of Acellular Pertussis Vaccine in a Mice Model

• Published in: Vaccines (Basel) Vol. 11; no. 5; p. 914
• Main Authors: Yang, Baifeng, Zhu, Dewu, Zhou, Yisi, Gong, Beizhe, Hu, Yuan, Zhang, Jiayou, Huang, Shihe, Nian, Xuanxuan, Li, Xinghang, Li, Xinguo, Duan, Kai, Yang, Xiaoming
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 28.04.2023; MDPI
• Subjects: adjuvant; Adjuvants; Aluminum; Aluminum hydroxide; Antibodies; Antigens; Bacterial infections; Bordetella pertussis; CD4 antigen; CD8 antigen; Cell-mediated immunity; Combined vaccines; Complications and side effects; Health aspects; Helper cells; Immunological memory; Infections; Laboratory animals; Liposomes; Lymphocytes T; mouse model; Neutralizing; Pathogens; Patient outcomes; Pertussis; Prevention; Respiration; tissue-resident memory T cell; vaccine; Vaccines; Whooping cough; China; United States > US; vaccine; Bordetella pertussis; mouse model; tissue-resident memory T cell; adjuvant
• ISSN: 2076-393X; 2076-393X
• DOI: 10.3390/vaccines11050914

The resurgence of pertussis in vaccinated communities may be related to the reduced long-term immunity induced by acellular pertussis vaccines. Therefore, developing improved pertussis vaccine candidates that could induce strong Th1 or Th17 cellular immunity is an urgent need. The use of new adjuvants may well meet this requirement. In this research, we developed a novel adjuvant candidate by combining liposome and QS-21 adjuvant. Adjuvant activity, protective efficacy, the level of neutralizing antibody against PT, and the resident memory T (T ) cells in lung tissue after vaccination were studied. We then performed respiratory challenge in mice after they received vaccination with traditional aluminum hydroxide and the novel adjuvant combination. Results showed that the liposome + QS-21 adjuvant group had a rapid antibody and higher antibody (PT, FHA, Fim) level, induced anti-PT neutralizing antibody and recruited more IL-17A-secreting CD4 T cells along with IL-17A-secreting CD8 T cells in mice, which provided robust protection against infection. These results provide a key basis for liposome + QS-21 adjuvant as a promising adjuvant candidate for developing an acellular pertussis vaccine that elicits protective immunity against pertussis.