Involvement of Adenosine in Vanadate-Stimulated Release of Lipoprotein Lipase Activity

• Published in: Biological & pharmaceutical bulletin Vol. 21; no. 9; pp. 889 - 892
• Main Authors: MOTOYASHIKI, Toshio, FUKAMACHI, Mika, MORITA, Tetsuo, SHIOMI, Hirohito, UEKI, Hiroshi
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.09.1998; Maruzen; Japan Science and Technology Agency
• Subjects: 5'-nucleotidase; 5'-Nucleotidase - antagonists & inhibitors; A1 receptor; Adenine - analogs & derivatives; Adenine - pharmacology; adenosine; Adenosine - biosynthesis; Adenosine - pharmacology; Adenosine Deaminase Inhibitors; Adenylyl Cyclases - metabolism; Adipose Tissue - drug effects; Adipose Tissue - enzymology; Animals; Biological and medical sciences; Enzyme Inhibitors - pharmacology; Extracellular Space - metabolism; General and cellular metabolism. Vitamins; In Vitro Techniques; lipoprotein lipase; Lipoprotein Lipase - metabolism; Male; Medical sciences; Pharmacology. Drug treatments; Rats; Rats, Wistar; Stimulation, Chemical; vanadate; Vanadates - pharmacology; Adipocyte; Adenosine; Adipose tissue; Rat; Enzyme; Rodentia; Cyclic AMP; Esterases; Lipoprotein lipase; Lipoprotein; Metabolism; Fatty acids; In vitro; Biological activity; Carboxylic ester hydrolases; Vertebrata; Mammalia; Enzymatic activity; Animal; Vanadates; Hydrolases
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.21.889

A short-term incubation of isolated rat fat pads with vanadate showed the stimulated release of lipoprotein lipase (LPL) activity and suppression of the rise in extracellular adenosine level. The addition of adenosine to the medium showed inhibition of both the stimulated release of LPL activity and an increase in intracellular cAMP content by vanadate. A progressive increase in 5'-nucleotidase activity in the particulate fraction containing plasma membrane was supressed by vanadate in a time- and dose-dependent manner, suggesting that vanadate inhibits, in part, the production of adenosine based on a dephosphorylation of AMP. In adipocytes, the inhibition of adenylate cyclase via A1 adenosine receptor is more predominant than the stimulation of adenylate cyclase via A2 adenosine receptor (Londos C. et al., Proc. Natl. Acad. Sci. U.S.A., 75, 5362-5366 (1978)).Therefore, vanadate may stimulate the release of LPL activity from the fat pads by suppressing the rise in extracellular adenosine level, accompanied by the activation of adenylate cyclase activity.