Functional study of the residue C899 in the 900 tetraloop of Escherichia coli small subunit ribosomal RNA

• Published in: Bioscience, biotechnology, and biochemistry Vol. 73; no. 11; pp. 2544 - 2546
• Main Authors: Ha, H.J., Chung-Ang Univ., Seoul (Korea R.), Song, W.S, Kim, H.M, Son, H.S, Lee, K
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01.11.2009; Japan Society for Bioscience Biotechnology and Agrochemistry; Oxford University Press
• Subjects: 900 tetraloop; ARN RIBOSOMAL; ARN RIBOSOMIAL; Biological and medical sciences; CHLORAMPHENICOL; CLORAMFENICOL; ESCHERICHIA COLI; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - chemistry; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Fundamental and applied biological sciences. Psychology; http://www.fao.org/aos/agrovoc#c_16014; http://www.fao.org/aos/agrovoc#c_33700; http://www.fao.org/aos/agrovoc#c_5013; http://www.fao.org/aos/agrovoc#c_6258; http://www.fao.org/aos/agrovoc#c_6596; http://www.fao.org/aos/agrovoc#c_6597; http://www.fao.org/aos/agrovoc#c_9817; intersubunit bridge; Mutagenesis; MUTANT; MUTANTES; MUTANTS; Nucleic Acid Conformation; Peptide Chain Initiation, Translational; PLASMIDE; PLASMIDIOS; PLASMIDS; PROTEIN SYNTHESIS; RIBOSOMAL RNA; RIBOSOMAS; RIBOSOME; Ribosome Subunits, Small - genetics; Ribosome Subunits, Small - metabolism; RIBOSOMES; RNA, Bacterial - chemistry; RNA, Bacterial - genetics; RNA, Bacterial - metabolism; RNA, Ribosomal, 16S - chemistry; RNA, Ribosomal, 16S - genetics; RNA, Ribosomal, 16S - metabolism; SINTESIS DE PROTEINAS; small subunit ribosomal RNA (SSU rRNA); specialized ribosome; subunit association; SYNTHESE PROTEIQUE; Ribosome; Ribosomal RNA; Residue; Escherichia coli; 900 tetraloop; subunit association; Bacteria; intersubunit bridge; specialized ribosome; Subunit; small subunit ribosomal RNA (SSU rRNA); Enterobacteriaceae
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.90460

A mutant ribosome bearing C899G in the 900 tetraloop of Escherichia coli 16S rRNA, one implicated in a conformational switch in the dynamic movements of the ribosome, showed defects in subunit association and 30S initiation complex formation. Our results explain the basis of the loss of protein synthesis ability caused by a perturbation of the 900 tetraloop.