The international collaborative study establishing the first international standard for timothy ( Phleum pratense) grass pollen allergenic extract

• Published in: Journal of allergy and clinical immunology Vol. 75; no. 2; pp. 258 - 267
• Main Authors: Gjesing, B., Jäger, L., Marsh, D.G., Løwenstein, H.
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.01.1985; Elsevier
• Subjects: Allergens - analysis; Allergens - standards; Antigens, allergens; Biological and medical sciences; Complement Activation; Dose-Response Relationship, Immunologic; Drug Stability; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Freeze Drying; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Histamine Release; Humans; Immediate hypersensitivity. Allergy. Anaphylaxis, etc; Immunobiology; Immunoelectrophoresis; International Cooperation; Isoelectric Focusing; Phleum pratense; Plant Extracts - analysis; Plant Extracts - standards; Poaceae - immunology; Pollen - immunology; Proteins - analysis; Radioallergosorbent Test; England; IS; IEF; QIE; RIE; SDS-PAGE; CRIE; IUIS; London; NIBSC; GCV; CIE; HPLC; WHO; Monocotyledones; Vegetals; Phleum pratense; Gramineae; Multicenter study; Angiospermae; Standardization; Spermatophyta; Extract; Pollen; Allergen
• ISSN: 0091-6749; 1097-6825
• DOI: 10.1016/0091-6749(85)90055-7

A selected candidate international reference preparation of timothy grass (Phleum pratense)-pollen extract was studied together with two other freeze-dried timothy pollen allergenic extracts in a multinational study. The collaborators used RAST inhibition, histamine release, quantitative immunoelectrophoresis (crossed immunoelectrophoresis/crossed radioimmunoelectrophoresis and rockets), isoelectric focusing, and other methods. The total allergenic potencies measured in RAST inhibition were evaluated for validity of linearity and parallel-line response. The relative concentrations of some important individual allergenic components were measured. The relative potencies for the total allergenic activity and the timothy components studied in each preparation were expressed relative to the selected candidate. This preparation was established in 1983 by the World Health Organization expert committee on biologic standardization as the international standard for timothy grass pollen extracts with assigned units of 100,000 IU per ampule.