Stem Cells from Human Exfoliated Deciduous Teeth Attenuate Atopic Dermatitis Symptoms in Mice through Modulating Immune Balance and Skin Barrier Function

• Published in: Mediators of inflammation Vol. 2022; pp. 1 - 11
• Main Authors: Xiong, Hao, Yang, Jin, Liu, Tao, Liu, Guangren, Han, Yongzhi, Dong, Xiuqin
• Format: Journal Article
• Language: English
• Published: New York Hindawi 21.07.2022; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: 1-Chloro-2,4-dinitrobenzene; Advertising executives; Atopic dermatitis; Cytokines; Dermatitis; Eczema; Edema; Erythema; Hay fever; Health aspects; Helper cells; Immunoglobulin E; Immunoglobulin G; Immunomodulation; Inflammatory diseases; Lymphocytes T; Mast cells; Mesenchyme; mRNA; Olive oil; Omalizumab; Polymerase chain reaction; Pruritus; Reagents; Rhinitis; RNA; Scratching behavior; Skin; Skin diseases; Skin lesions; Stem cell transplantation; Stem cells; Teeth; Thymic stromal lymphopoietin; Thymus; Tumor necrosis factor-TNF; China; United States > US; Japan
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/2022/6206883

Atopic dermatitis (AD) is a chronic skin inflammatory disease associated with immune abnormalities and disrupted skin barrier function. Mesenchymal stem cells (MSCs) have been suggested as an alternative therapeutic option in AD. Stem cells from human exfoliated deciduous teeth (SHEDs) are a unique postnatal stem cell population with high immunomodulatory properties. The aim of this study was to explore the effects of SHEDs on AD in the BALB/c mouse model induced by 2,4-dinitrochlorobenzene (DNCB). SHEDs were administrated intravenously or subcutaneously, and clinical severity, histopathological findings, skin barrier function, and organ indexes were evaluated. Skin tissue cytokine mRNA levels and serum cytokine protein levels were further analysed. SHED administration significantly alleviated AD clinical severity, including dermatitis scores, ear thickness, scratching behaviour, and infiltration of mast cells. In addition, disrupted skin barrier function and enlarged spleens were restored by SHED administration. Further, SHED treatment reduced the levels of IgE, IgG1, and thymic stromal lymphopoietin (TSLP) in the serum and the modulated expression of Th1-, Th2-, and Th17-associated cytokines in skin lesions. In conclusion, SHEDs attenuated AD-like skin lesions in mice by modulating the immune balance and skin barrier function. SHEDs could be a potential new treatment agent for AD.