Development of designed site-directed pseudopeptide-peptido-mimetic immunogens as novel minimal subunit-vaccine candidates for malaria

• Published in: Molecules (Basel, Switzerland) Vol. 15; no. 12; pp. 8856 - 8889
• Main Authors: Lozano, José Manuel, Lesmes, Liliana P, Carreño, Luisa F, Gallego, Gina M, Patarroyo, Manuel Elkin
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 06.12.2010; MDPI
• Subjects: Animals; Antigens; Asymmetry; Blood; Carbon; Haplorhini; Humans; Ig passive transferring; Immunoglobulins; Infectious diseases; Malaria; malaria vaccine; Malaria Vaccines - chemical synthesis; Malaria Vaccines - chemistry; Malaria Vaccines - immunology; Malaria Vaccines - pharmacology; Malaria, Falciparum - immunology; Malaria, Falciparum - prevention & control; Mice; Mice, Inbred BALB C; NMR; Nuclear magnetic resonance; Pathogens; peptide high binding motif; peptide-bond isoster; Peptides; Peptidomimetics - chemical synthesis; Peptidomimetics - chemistry; Peptidomimetics - immunology; Peptidomimetics - pharmacology; Proteins; pseudopeptide; transition-state analogue; Vaccines; Vaccines, Subunit - chemical synthesis; Vaccines, Subunit - chemistry; Vaccines, Subunit - immunology; Vaccines, Subunit - pharmacology; Vaccines, Synthetic - chemistry; Vaccines, Synthetic - immunology; Vaccines, Synthetic - pharmacology; Colombia
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules15128856

Synthetic vaccines constitute the most promising tools for controlling and preventing infectious diseases. When synthetic immunogens are designed from the pathogen native sequences, these are normally poorly immunogenic and do not induce protection, as demonstrated in our research. After attempting many synthetic strategies for improving the immunogenicity properties of these sequences, the approach consisting of identifying high binding motifs present in those, and then performing specific changes on amino-acids belonging to such motifs, has proven to be a workable strategy. In addition, other strategies consisting of chemically introducing non-natural constraints to the backbone topology of the molecule and modifying the α-carbon asymmetry are becoming valuable tools to be considered in this pursuit. Non-natural structural constraints to the peptide backbone can be achieved by introducing peptide bond isosters such as reduced amides, partially retro or retro-inverso modifications or even including urea motifs. The second can be obtained by strategically replacing L-amino-acids with their enantiomeric forms for obtaining both structurally site-directed designed immunogens as potential vaccine candidates and their Ig structural molecular images, both having immuno-therapeutic effects for preventing and controlling malaria.