Isolation and characterization of human lung cancer antigens by serological screening with autologous antibodies

• Published in: Cancer letters Vol. 301; no. 1; pp. 57 - 62
• Main Authors: Hanafusa, Tadashi, Mohamed, Ali Eldib Ali, Kitaoka, Kenta, Ohue, Yoshihiro, Nakayama, Eiichi, Ono, Toshiro
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier Ireland Ltd 01.02.2011; Elsevier Limited
• Subjects: adenocarcinoma; Adenocarcinoma - immunology; antibodies; Antibodies, Neoplasm - immunology; antigens; Antigens, Neoplasm - genetics; Antigens, Neoplasm - immunology; Antigens, Neoplasm - isolation & purification; Cell Line, Tumor; clones; Cloning; Deoxyribonucleic acid; development aid; DNA; Gene expression; gene expression regulation; Gene Library; genes; Hematology, Oncology and Palliative Medicine; Humans; Lung adenocarcinoma; Lung cancer; lung neoplasms; Lung Neoplasms - immunology; microarray technology; MYL6B; NUP107; patients; Proteins; screening; SEREX; Tumor antigen; SEREX; MYL6B; NUP107; Lung adenocarcinoma; Tumor antigen
• ISSN: 0304-3835; 1872-7980
• DOI: 10.1016/j.canlet.2010.10.024

Abstract Serological analysis of a recombinant cDNA expression library (SEREX) derived from two lung adenocarcinoma cancer cell lines using autologous sera led to the isolation of 41 positive cDNA clones comprising 28 different antigens. They coded for a variety of nuclear and cytoplasmic proteins. Among the antigens, nucleoporin 107 (NUP107) was isolated most frequently (5 of 41 clones). The second most frequently isolated antigen was coded for by C21orf58 (4 of 41 clones). During serological analysis of selected antigens based on their reactivity to sera from normal individuals and lung cancer patients, none of the antigens showed a cancer-restricted recognition pattern. However, five genes including NUP107 showed higher expression when we examined the changes in gene expression in five different adenocarcinoma cell lines, including those used in SEREX, compared with their levels in normal lung tissues by cDNA microarray analysis. On the other hand, the expression levels of five genes including C21orf58 were down regulated in all adenocarcinoma cell lines. This SEREX study combining comprehensive gene expression assays has added to the growing list of lung cancer antigens, which may aid the development of diagnostic and immunotherapeutic reagents for patients with lung cancer.