Some pitfalls in PAGE-LA-ICP-MS for quantitative elemental speciation of dissolved organic matter and metalomics

• Published in: Analytical & bioanalytical chemistry (Print) Vol. 393; no. 2; pp. 699 - 707
• Main Authors: Jiménez, Maria S, Gomez, Maria T, Rodriguez, L, Martinez, L, Castillo, Juan R
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.01.2009; Springer-Verlag; Springer
• Subjects: Analytical Chemistry; Applied sciences; Binding; Biochemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Copper; Copper - analysis; Copper - metabolism; Electrophoresis; Electrophoresis, Polyacrylamide Gel; Elemental speciation; Exact sciences and technology; Food Science; gel electrophoresis; Global environmental pollution; Hydrocarbons, Iodinated - analysis; Hydrocarbons, Iodinated - metabolism; Laboratory Medicine; Laser ablation inductively coupled plasma mass spectrometry; Metal binding to natural organic matter; Metal-binding proteins; metalloproteins; Metalloproteins - analysis; Metalloproteins - metabolism; Monitoring/Environmental Analysis; Original Paper; Pollution; Sensitivity and Specificity; Sodium; Speciation; Spectrometric and optical methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfates; Superoxide dismutase; Thyroglobulin - analysis; Thyroglobulin - metabolism; Zinc; Zinc - analysis; Zinc - metabolism; Metal-binding proteins; Elemental speciation; Metal binding to natural organic matter; Gel electrophoresis; Laser ablation inductively coupled plasma mass spectrometry; Metalloproteins; Organic matter; Coupled method; Metal complex; Sodium compound; Inductive coupling plasma spectrometry; Protein; Lauryl sulfate; Chemical enrichment; Acrylamide polymer; Environment; Laser ablation technique; Leachate; Mass spectrometry; Laser ablation inductively coupled plasma mass spectrometry Elemental speciation; Speciation; Iodine
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-008-2384-2

An experimental approach to evaluate the capabilities and limitations of polyacrylamide gel electrophoresis (PAGE) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) for quantitative elemental speciation is presented. Two metalloproteins (superoxide dismutase, containing Cu and Zn, and thyroglobulin, containing I) with high binding affinity for metals, and metal-dissolved organic matter (DOM) complexes (from a compost leachate sample) which show different types of metal binding are studied. Iodine can be quantitatively detected in thyroglobulin after PAGE-LA-ICP-MS using either sodium dodecyl sulfate (SDS) PAGE or native PAGE. However, detection of Cu and Zn in superoxide dismutase after PAGE-LA-ICP-MS depends on the conditions of the PAGE method because possible metal losses can occur (either with SDS-PAGE or with native PAGE). The use of PAGE-LA-ICP-MS to study the contribution of DOM to the mobilization of metals from environmental samples is possible, but it depends also on the PAGE separation conditions owing to disequilibrium effects of metal-DOM complexes.