Induction of cytokines and ICAM-1 by proinflammatory cytokines in primary rheumatoid synovial fibroblasts and inhibition by N-acetyl-L-cysteine and aspirin
The role of transcription factor NF-kB in the induction of cytokines and ICAM-1 upon stimulation with proinflammatory cytokines, IL-1 and tumor necrosis factor (TNF)-α was investigated in primary synovial fibroblasts obtained from patients with rheumatoid arthritis (RA). Nuclear translocation of NF-...
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Published in | International immunology Vol. 8; no. 10; pp. 1483 - 1493 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
England
Oxford University Press
01.10.1996
Oxford Publishing Limited (England) |
Subjects | |
Online Access | Get full text |
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Summary: | The role of transcription factor NF-kB in the induction of cytokines and ICAM-1 upon stimulation with proinflammatory cytokines, IL-1 and tumor necrosis factor (TNF)-α was investigated in primary synovial fibroblasts obtained from patients with rheumatoid arthritis (RA). Nuclear translocation of NF-kB was demonstrated after 30 min of treatment with IL-1 or TNF-α. Thereafter, the production of several cytokines including granulocyte macrophage colony stimulating factor, IL-6 and IL-8, that are known to be abundantly produced in the synovial cavity of RA patients, was greatly augmented. Similarly, cell surface expression of ICAM-1 was induced by the IL-1 or TNF-α treatment. Since expression of these genes is induced in rheumatoid synovial tissue, this experimental system is considered to represent the in vivo situation of RA pathophysiology. Using this cell culture system we attempted to modulate the intracellular signaling cascade for NF-kB activation and examined the effects of N-acetyl-L-cysteine (NAC) and acetylsalicylic acid (aspirin), which were previously reported to inhibit NF-kB activation. Pretreatment of the primary synovial fibroblasts with NAC inhibited nuclear translocation of NF-kB. Subsequently, the induction of these cytokines and ICAM-1 was considerably suppressed. On the other hand, pretreatment with aspirin blocked these phenomena only partially. These observations indicate the pivotal role of NF-kB in RA pathogenesis thus highlighting the possibility of a novel therapeutic strategy. |
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Bibliography: | ark:/67375/HXZ-Q6BFHJ52-0 ArticleID:8.10.1483 istex:D580A0B880B5FC6C12074C0CF3119ED8872C47EE 1Present address Division of Immunology, Institute of Gerontology, Nippon Medical School, 1–396 Kosugi-cho, Nakahara-ku, Kawasaki 211, Japan ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0953-8178 1460-2377 |
DOI: | 10.1093/intimm/8.10.1483 |