Regulation of inflammatory cytokines in lipopolysaccharide-stimulated RAW 264.7 murine macrophage by 7-O-methyl-naringenin

• Published in: Molecules (Basel, Switzerland) Vol. 17; no. 3; pp. 3574 - 3585
• Main Authors: Soromou, Lanan Wassy, Zhang, Zhichao, Li, Rongtao, Chen, Na, Guo, Weixiao, Huo, Meixia, Guan, Shuang, Lu, Jing, Deng, Xuming
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.03.2012; MDPI
• Subjects: 7-O-methylnaringenin; Animals; anti-inflammation; Cell Line; Cytokines; Cytokines - metabolism; Flavanones - chemistry; Flavanones - pharmacology; Inflammation; Interleukin-1beta - metabolism; Interleukin-6 - metabolism; Investigations; Kinases; Lipopolysaccharides - pharmacology; Macrophages - drug effects; Macrophages - metabolism; Mice; Phosphorylation; Polyphenols; signal pathways; Signal transduction; Tumor Necrosis Factor-alpha - metabolism; Tumor necrosis factor-TNF; China
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules17033574

7-O-Methylnaringenin, extracted from Rhododendron speciferum, belongs to the flavanone class of polyphenols. In the present study, we investigated the anti-inflammatory effects of 7-O-methylnaringenin on cytokine production by lipopoly-saccharide (LPS)-stimulated RAW 264.7 macrophages in vitro. The results showed that pretreatment with 10, 20 or 40 μg/mL of 7-O-methylnaringenin could downregulate tumour necrosis factor (TNF-α), interleukin (IL-6) and interleukin (IL-1β) in a dose-dependent manner. Furthermore, we investigated the signal transduction mechanisms to determine how 7-O-methylnaringenin affects RAW 264.7 macrophages. The activation of mitogen-activated protein kinases (MAPK) and IκBα were measured by Western blotting. The data showed that 7-O-methylnaringenin could downregulate LPS-induced levels of phosphorylation of ERK1/2, JNK and IκBα. These observations indicated that 7-O-methylnaringenin modulated inflammatory cytokine responses by blocking NF-қB, ERK1/2 and JNK/MAPKs activation.