A Novel Anti-Allergic Drug, Betotastine Besilate, Suppresses Interleukin-5 Production by Human Peripheral Blood Mononuclear Cells

• Published in: Biological & pharmaceutical bulletin Vol. 21; no. 4; pp. 411 - 413
• Main Authors: KAMINUMA, Osamu, OGAWA, Koji, KIKKAWA, Hideo, KIKUCHI, Matsuo, NAITO, Kazuaki, IKEZAWA, Katsuo
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 01.04.1998; Maruzen; Japan Science and Technology Agency
• Subjects: Adult; allergy; Anti-Allergic Agents - pharmacology; atopy; betotastine besilate; Biological and medical sciences; Cell Division - drug effects; Cells, Cultured; eosinophil; Histamine and antagonists. Allergy; Humans; IL-5; Indicators and Reagents; Interleukin-5 - biosynthesis; Ketotifen - pharmacology; Male; Medical sciences; Middle Aged; Monocytes - drug effects; Monocytes - metabolism; Pharmacology. Drug treatments; Piperidines - pharmacology; Pyridines - pharmacology; T cell; Human; Cell proliferation; Immunopathology; Allergy; Cytokine; Inflammation; Normal; In vitro; Biological activity; Antiallergic; Eosinophil; Atopy; Interleukin 5; Mononuclear cell; T-Lymphocyte; Mechanism of action
• ISSN: 0918-6158; 1347-5215
• DOI: 10.1248/bpb.21.411

The effect of a novel anti-allergic drug, betotastine besilate (betotasitine) on intertleukin (IL)-5 production by human peripheral blood mononuclear cells (PBMC) was investigated. PBMC of Dermatophagoides farinae extract (Df)-sensitive donors produced IL-5 and showed a proliferative responce upon stimulation with relevant antigen (10μg/ml). Df-induced IL-5 production by PBMC was significantly inhibited by betotastine at 10 and 100 μM. Betotastine also suppressed proliferation of PBMC with less potency. The effect of betotastine on IL-5 production was enhanced and significant even at 0.1 μM when the drug was added 120 min before antigen stimulation. Ketotifen and cetirizine also inhibited IL-5 production, but the effects of these drugs were significant only at 100 μM. These findings indicate that the suppression of IL-5 production may be involved in the anti-allergic effect of betotastine.