Production of cloned transgenic cow expressing omega-3 fatty acids

• Published in: Transgenic research Vol. 21; no. 3; pp. 537 - 543
• Main Authors: Wu, Xia, Ouyang, Hongsheng, Duan, Biao, Pang, Daxin, Zhang, Li, Yuan, Ting, Xue, Lian, Ni, Daibang, Cheng, Lei, Dong, Shuhua, Wei, Zhuying, Li, Lin, Yu, Ming, Sun, Qing-Yuan, Chen, Da-Yuan, Lai, Liangxue, Dai, Yifan, Li, Guang-Peng
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer-Verlag 01.06.2012; Springer Netherlands; Springer; Springer Nature B.V
• Subjects: Animal Genetics and Genomics; Animals; Animals, Genetically Modified; Animals, Genetically Modified - genetics; Animals, Genetically Modified - metabolism; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biotechnology; Blotting, Southern; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans Proteins; Caenorhabditis elegans Proteins - genetics; Caenorhabditis elegans Proteins - metabolism; Cattle; cows; cytology; desaturase; Domestic animals; Economics; Embryo Transfer; Fatty Acid Desaturases; Fatty Acid Desaturases - genetics; Fatty Acid Desaturases - metabolism; Fatty acids; Fatty Acids, Omega-3; Fatty Acids, Omega-3 - genetics; Fatty Acids, Omega-3 - metabolism; Female; Fundamental and applied biological sciences. Psychology; genes; Genes, Helminth; Genetic Engineering; Genetic technics; Genetic Vectors; Genetic Vectors - genetics; Genetic Vectors - metabolism; genetics; Life Sciences; metabolism; Methods. Procedures. Technologies; Milk; Milk - metabolism; Molecular Medicine; Nuclear Transfer Techniques; omega-3 fatty acids; Oocytes; Oocytes - cytology; Oocytes - metabolism; Original Paper; Plant Genetics and Genomics; Polyunsaturated fatty acids; Pregnancy; stearoyl-CoA desaturase; tissue analysis; Transfection; Transgenic animals; Transgenic animals and transgenic plants; Transgenics; Nuclear transfer; N-3 fatty acid desaturase gene; mfat-1; Transgenic cattle; Ox; Cow; n-3 fatty acid; Vertebrata; Mammalia; Gene; Production; Transfer; Livestock; Artiodactyla; Genetically modified organism; Transgenesis; Ungulata
• ISSN: 0962-8819; 1573-9368; 1573-9368
• DOI: 10.1007/s11248-011-9554-2

n-3 Polyunsaturated fatty acids (n-3 PUFA) are important for human health. Alternative resources of n-3 PUAFs created by transgenic domestic animals would be an economic approach. In this study, we generated a mfat-1 transgenic cattle expressed a Caenorhabditis elegans gene, mfat-1, encoding an n-3 fatty acid desaturase. Fatty acids analysis of tissue and milk showed that all of the examined n-3 PUAFs were greatly increased and simultaneously the n-6 PUAFs decreased in the transgenic cow. A significantly reduction of n-6/n-3 ratios (P < 0.05) in both tissue and milk were observed.