An Outbreak of Acute Hemorrhagic Conjunctivitis Caused by Coxsackievirus A24 in Eastern Uttar Pradesh, India 2023

• Published in: Intervirology Vol. 67; no. 1; pp. 106 - 113
• Main Authors: Behera, Sthita Pragnya, Mishra, Nalini, Yadav, Ramyash, Shukla, Aishwarya, Kumari, Moni, Rajput, Sonal, Fatma, Imbisat, Tiwari, Ashutosh, Srivastava, Prashansha, Tiwari, Shashikant, Singh, Rajeev, Ranawade, Satish S., Murhekar, Manoj, Dwivedi, Gaurav Raj
• Format: Journal Article
• Language: English
• Published: Basel, Switzerland S. Karger AG 01.01.2024; Karger Publishers
• Subjects: 3C Viral Proteases; acute hemorrhagic conjunctivitis; Adolescent; Adult; Amino Acid Substitution; Amino acids; Child; Child, Preschool; Conjunctivitis; Conjunctivitis, Acute Hemorrhagic - epidemiology; Conjunctivitis, Acute Hemorrhagic - virology; coxsackie virus a24; Coxsackievirus infections; Coxsackievirus Infections - epidemiology; Coxsackievirus Infections - virology; Disease Outbreaks; enterovirus; Enterovirus C, Human - classification; Enterovirus C, Human - genetics; Enterovirus C, Human - isolation & purification; Epidemiology; Female; Genotype; Health aspects; Humans; India - epidemiology; Male; Middle Aged; Molecular Epidemiology; outbreak; Phylogeny; Proteases; Research Article; Viral Proteins - genetics; Young Adult; India; China; Pakistan; Acute hemorrhagic conjunctivitis; Enterovirus; Outbreak; Conjunctivitis; Coxsackie virus A24
• ISSN: 0300-5526; 1423-0100; 1423-0100
• DOI: 10.1159/000540952

Abstract Introduction: Acute hemorrhagic conjunctivitis (AHC) outbreaks are caused mostly by viruses. During July-August 2023, there was a sudden spike in acute hemorrhage conjunctivitis cases in Eastern Uttar Pradesh, India. To identify the etiological and gain molecular epidemiology of the agent, the study was conducted. Methodology: Conjunctival swabs were collected from patients (n = 128) with presumed acute hemorrhage conjunctivitis visiting two tertiary care hospitals. Results: Enteroviruses infection was identified in 96 (75%) patients. In these patients, coxsackievirus A24 (CV-A24) infection was further confirmed by targeting the genetic regions of 3C protease and VP1. Furthermore, the study established the outbreak was caused by the genotype IV of CV-A24 with the highest genetic similarity with CV-A24 reported from Northeast India, China, and Pakistan circulating during the same period. The comparison of our study sequences with earlier Indian outbreak strains (2007) revealed four amino acid substitutions at the 3C region (“S21N,” “V30I,” “S66I,” and “V75I”) and three non-synonymous mutations at the VP1 region (“L16I,” “P21S,” and “N301D”). Conclusion: The study findings revealed that the AHC outbreak was caused by genotype IV of CV-A24 in this region. Molecular identification accompanied by phylogenetic analysis will be useful in studying the enterovirus epidemiology associated with AHC outbreaks.