Selection of transformation-efficient barley genotypes based on TFA (transformation amenability) haplotype and higher resolution mapping of the TFA loci
Key message The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. Barley ( Hordeum vulgare ) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars f...
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Published in | Plant cell reports Vol. 36; no. 4; pp. 611 - 620 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Berlin/Heidelberg
Springer Berlin Heidelberg
01.04.2017
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Key message
The genetic substitution of transformation amenability alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars.
Barley (
Hordeum vulgare
) cv. ‘Golden Promise’ is one of the most useful and well-studied cultivars for genetic manipulation. In a previous report, we identified several transformation amenability (
TFA
) loci responsible for
Agrobacterium
-mediated transformation using the F
2
generation of immature embryos, derived from ‘Haruna Nijo’ × ‘Golden Promise,’ as explants. In this report, we describe higher density mapping of these
TFA
regions with additional SNP markers using the same transgenic plants. To demonstrate the robustness of transformability alleles at the
TFA
loci, we genotyped 202 doubled haploid progeny from the cross ‘Golden Promise’ × ‘Full Pint.’ Based on SNP genotype, we selected lines having ‘Golden Promise’ alleles at
TFA
loci and used them for transformation. Of the successfully transformed lines, DH120366 came the closest to achieving a level of transformation efficiency comparable to ‘Golden Promise.’ The results validate that the genetic substitution of
TFA
alleles from ‘Golden Promise’ can facilitate the development of transformation-efficient lines from recalcitrant barley cultivars. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0721-7714 1432-203X |
DOI: | 10.1007/s00299-017-2107-2 |