Replication-Dependent Marking of DNA by PCNA Facilitates CAF-1-Coupled Inheritance of Chromatin

Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for C...

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Bibliographic Details
Published inCell Vol. 96; no. 4; pp. 575 - 585
Main Authors Shibahara, Kei-ichi, Stillman, Bruce
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 19.02.1999
Subjects
Adenosine Triphosphate - pharmacology
Autoantibodies - pharmacology
Cell Division - genetics
Chromatin - genetics
Chromatin - metabolism
Chromatin Assembly Factor-1
Chromosomal Proteins, Non-Histone
DNA Replication - drug effects
DNA Replication - genetics
DNA, Superhelical - drug effects
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
DNA-Binding Proteins - pharmacology
HeLa Cells
Homeodomain Proteins
Humans
Minor Histocompatibility Antigens
Proliferating Cell Nuclear Antigen - genetics
Proliferating Cell Nuclear Antigen - immunology
Proliferating Cell Nuclear Antigen - metabolism
Protein Binding - genetics
Proto-Oncogene Proteins c-bcl-2
Replication Protein C
Repressor Proteins
Saccharomyces cerevisiae Proteins
Simian virus 40 - genetics
Temperature
Transcription Factors
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Summary:Chromatin assembly factor 1 (CAF-1) is required for inheritance of epigenetically determined chromosomal states in vivo and promotes assembly of chromatin during DNA replication in vitro. Herein, we demonstrate that after DNA replication, replicated, but not unreplicated, DNA is also competent for CAF-1-dependent chromatin assembly. The proliferating cell nuclear antigen (PCNA), a DNA polymerase clamp, is a component of the replication-dependent marking of DNA for chromatin assembly. The clamp loader, replication factor C (RFC), can reverse this mark by unloading PCNA from the replicated DNA. PCNA binds directly to p150, the largest subunit of CAF-1, and the two proteins colocalize at sites of DNA replication in cells. We suggest that PCNA and CAF-1 connect DNA replication to chromatin assembly and the inheritance of epigenetic chromosome states.
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ISSN:0092-8674
1097-4172
DOI:10.1016/S0092-8674(00)80661-3