Isolation and Characterization of a Purple Non-Sulfur Photosynthetic Bacterium Rhodopseudomonas faecalis Strain A from Swine Sewage Wastewater

• Published in: Biocontrol Science Vol. 21; no. 1; pp. 29 - 36
• Main Authors: WEI, HONGYI, OKUNISHI, SUGURU, YOSHIKAWA, TAKESHI, KAMEI, YUTO, MAEDA, HIROTO
• Format: Journal Article
• Language: English
• Published: Japan The Society for Antibacterial and Antifungal Agents, Japan 2016; Japan Science and Technology Agency
• Subjects: 16S rDNA; Ammonia - chemistry; Animals; Bacteria; Nitrogen - chemistry; Photosynthesis; Phylogeny; Purple non-sulfur photosynthetic bacterium; Rhodopseudomonas; Rhodopseudomonas - classification; Rhodopseudomonas - genetics; Rhodopseudomonas - isolation & purification; Rhodopseudomonas - metabolism; Rhodopseudomonas faecalis; RNA, Ribosomal, 16S - genetics; Sewage - analysis; Sewage - microbiology; Sulfur - metabolism; Swine; Swine sewage wastewater
• ISSN: 1342-4815; 1884-0205
• DOI: 10.4265/bio.21.29

A purple non-sulfur photosynthetic bacterium (PNSB), PSB Strain A was isolated from swine sewage wastewater. Phylogenetic analysis revealed that PSB Strain A was most closely related to Rhodopseudomonas faecalis. Growth of the isolate under anaerobic-light conditions with a variety of carbon sources was investigated. Both PSB Strain A and the standard strain showed good growth with acetic acid, propionic acid, and n-butyric acid at a concentration of 20 mM. At the high concentration of 200 mM, PSB Strain A showed better growth in pyruvate, acetate, propionate, succinate and malate. By applying PSB Strain A to treat swine sewage wastewater, the concentration of VFAs, which were acetic acid and propionic acid, decreased from 158.0 mM to 120.2±2.9 mM, and 14.9 mM to 9.3±0.9 mM, respectively, after 216-h incubation. After 330-h incubation, the concentrations of TOC and ammonia nitrogen dropped from 4508.0 mg/L to 3104.0±451.5 mg/L, and 629.7 mg/L to 424.1±7.4 mg/L, respectively. The isolated PSB Strain A showed almost the same efficiency compared with the standard strain on the removal of VFAs and TOC. The results suggest the possibility of using the isolated strain to treat swine sewage wastewater.