Palm oil utilization for the simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa

• Published in: Applied microbiology and biotechnology Vol. 78; no. 6; pp. 955 - 961
• Main Authors: Marsudi, Sidik, Unno, Hajime, Hori, Katsutoshi
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2008; Springer Berlin Heidelberg; Springer; Springer Nature B.V
• Subjects: Biological and medical sciences; Biomass; Bioreactors; Biotechnological Products and Process Engineering; Biotechnology; Carbon; Cell growth; Chemical engineering; Chemical synthesis; Fatty acids; Fermentation; Fundamental and applied biological sciences. Psychology; Glycerol; Glycerol - metabolism; Glycolipids - metabolism; Life Sciences; Methods. Procedures. Technologies; Microbial engineering. Fermentation and microbial culture technology; Microbial Genetics and Genomics; Microbiology; Microorganisms; Oils & fats; Oleic Acid - metabolism; Palm Oil; palm oils; Plant Oils - chemistry; Plant Oils - metabolism; Polyhydroxyalkanoates (PHAs); Polyhydroxyalkanoates - chemistry; Polyhydroxyalkanoates - metabolism; Pseudomonas aeruginosa; Pseudomonas aeruginosa - metabolism; Pseudomonas aeruginosa - ultrastructure; Rhamnolipid; Studies; Triacylglycerol; triacylglycerol lipase; Vegetable oils; Palm oil; Rhamnolipid; Triacylglycerol; Lipase; Polyhydroxyalkanoates (PHAs); Pseudomonadales; Enzyme; Ester polymer; Alkanoate(hydroxy) polymer; Triacylglycerol lipase; Esterases; Carboxylic ester hydrolases; Glycolipid; Biosurfactant; Rhamnolipid· Pseudomonas aeruginosa, Palm oil; Triacylglycerol· Lipase; Production; Microorganism culture; Bacteria; Hydrolases; Pseudomonadaceae; Pseudomonas aeruginosa
• ISSN: 0175-7598; 1432-0614
• DOI: 10.1007/s00253-008-1388-3

Direct utilization of palm oil for the simultaneous production of polyhydroxyalkanoates (PHAs) and rhamnolipids was demonstrated using Pseudomonas aeruginosa IFO3924. By secreted lipase, palm oil was hydrolyzed into glycerol and fatty acids. Fatty acids became favorable carbon sources for cell growth and PHA production via β-oxidation and glycerol for rhamnolipid production via de novo fatty acid synthesis. Both PHA and rhamnolipid syntheses started after the nitrogen source was exhausted and cell growth ceased. PHA synthesis continued until all fatty acids were exhausted, and at that time, PHA content in the cells reached a maximum, but stopped despite the remaining glycerol (<2g/l). In contrast, rhamnolipid synthesis continued until glycerol was exhausted.