ADF/Cofilin-Mediated Actin Turnover Promotes Axon Regeneration in the Adult CNS
Injured axons fail to regenerate in the adult CNS, which contrasts with their vigorous growth during embryonic development. We explored the potential of re-initiating axon extension after injury by reactivating the molecular mechanisms that drive morphogenetic transformation of neurons during develo...
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Published in | Neuron (Cambridge, Mass.) Vol. 103; no. 6; pp. 1073 - 1085.e6 |
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Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
25.09.2019
Elsevier Limited |
Subjects | |
Online Access | Get full text |
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Summary: | Injured axons fail to regenerate in the adult CNS, which contrasts with their vigorous growth during embryonic development. We explored the potential of re-initiating axon extension after injury by reactivating the molecular mechanisms that drive morphogenetic transformation of neurons during development. Genetic loss- and gain-of-function experiments followed by time-lapse microscopy, in vivo imaging, and whole-mount analysis show that axon regeneration is fueled by elevated actin turnover. Actin depolymerizing factor (ADF)/cofilin controls actin turnover to sustain axon regeneration after spinal cord injury through its actin-severing activity. This pinpoints ADF/cofilin as a key regulator of axon growth competence, irrespective of developmental stage. These findings reveal the central role of actin dynamics regulation in this process and elucidate a core mechanism underlying axon growth after CNS trauma. Thereby, neurons maintain the capacity to stimulate developmental programs during adult life, expanding their potential for plasticity. Thus, actin turnover is a key process for future regenerative interventions.
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•Elevated actin turnover is essential for regenerative growth•ADF/cofilin activity increases during conditioning-mediated regeneration•ADF/cofilin is necessary and sufficient for axon regeneration•The severing activity of ADF/cofilin is critical for axon regeneration
Tedeschi et al. identify ADF/cofilin as a key driver of axon regeneration in adult dorsal root ganglion neurons. Specifically, enhanced actin turnover by the ADF/cofilin severing function controls axon regeneration in the adult CNS. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Undefined-1 ObjectType-Feature-3 content type line 23 C.L., K.F., and F.B. conceived the project; A.T., S.D., M.C., C.L., B.S., and F.B. designed research; A.T., S.D., M.C., C.L., B.S., K.F., T.S., and S.S. performed research; A.T., S.D., M.C., C.L., B.S., K.F., T.S., S.S., M.L., and B.H. analyzed the data; C.G. and W.W. provided mutant mice and antibodies; F.B. supervised the research; A.T.,S.D., M.C., C.L., B.S., and F.B wrote the paper. T.S., S.S., B.J.H., C.G., and W.W. provided feedback and contributed to editing the manuscript. These authors contributed equally. AUTHOR CONTRIBUTIONS |
ISSN: | 0896-6273 1097-4199 |
DOI: | 10.1016/j.neuron.2019.07.007 |