Flowering-Related RING Protein 1 (FRRP1) Regulates Flowering Time and Yield Potential by Affecting Histone H2B Monoubiquitination in Rice (Oryza Sativa)

• Published in: PloS one Vol. 11; no. 3; p. e0150458
• Main Authors: Du, Yiwei, He, Wei, Deng, Changwang, Chen, Xi, Gou, Lanming, Zhu, Fugui, Guo, Wei, Zhang, Jianfu, Wang, Tao
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.03.2016; Public Library of Science (PLoS)
• Subjects: Agricultural production; Agriculture; Agronomy; Amino Acid Sequence; Arabidopsis; Biology and Life Sciences; Cell cycle; Crop yield; Crop yields; Flowering; Flowers - chemistry; Flowers - genetics; Flowers - physiology; Gene expression; Gene Expression Regulation, Plant; Genetic aspects; Germplasm; Grain; Histone H2B; Histones - chemistry; Histones - genetics; Histones - metabolism; Laboratories; Measurement; Molecular Sequence Data; Oryza - chemistry; Oryza - genetics; Oryza - physiology; Oryza sativa; Physiology; Plant flowering; Plant Proteins - chemistry; Plant Proteins - genetics; Plant Proteins - metabolism; Plant sciences; Plants, Genetically Modified - chemistry; Plants, Genetically Modified - genetics; Plants, Genetically Modified - physiology; Proteins; Research and Analysis Methods; Rice; RNA Interference; RNA-mediated interference; Sequence Alignment; Transgenic plants; Trends; Ubiquitin-protein ligase; Ubiquitination
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0150458

Flowering time is a critical trait for crops cultivated under various temperature/photoperiod conditions around the world. To understand better the flowering time of rice, we used the vector pTCK303 to produce several lines of RNAi knockdown transgenic rice and investigated their flowering times and other agronomic traits. Among them, the heading date of FRRP1-RNAi knockdown transgenic rice was 23-26 days earlier than that of wild-type plants. FRRP1 is a novel rice gene that encodes a C3HC4-type Really Interesting Novel Gene (RING) finger domain protein. In addition to the early flowering time, FRRP1-RNAi knockdown transgenic rice caused changes on an array of agronomic traits, including plant height, panicle length and grain length. We analyzed the expression of some key genes associated with the flowering time and other agronomic traits in the FRRP1-RNAi knockdown lines and compared with that in wild-type lines. The expression of Hd3a increased significantly, which was the key factor in the early flowering time. Further experiments showed that the level of histone H2B monoubiquitination (H2Bub1) was noticeably reduced in the FRRP1-RNAi knockdown transgenic rice lines compared with wild-type plants and MBP-FRRP1-F1 was capable of self-ubiquitination. The results indicate that Flowering Related RING Protein 1 (FRRP1) is involved in histone H2B monoubiquitination and suggest that FRRP1 functions as an E3 ligase in vivo and in vitro. In conclusion, FRRP1 probably regulates flowering time and yield potential in rice by affecting histone H2B monoubiquitination, which leads to changes in gene expression in multiple processes.