A one-step multiplex PCR to identify Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae in the clinical routine

Abstract Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, LEN and OKP and their flanking gene ( deo R). Since this scheme focus...

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Published inDiagnostic microbiology and infectious disease Vol. 87; no. 4; pp. 315 - 317
Main Authors Fonseca, Erica Lourenço, da Veiga Ramos, Nilceia, Nascimento Andrade, Bruno G, Morais, Lena L.C.S, Abanto Marin, Michel F, Vicente, Ana Carolina P
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.04.2017
Subjects
beta-Lactamases - genetics
blaLEN
blaOKP
blaSHV
Class A β-lactamase
DNA, Bacterial - genetics
Humans
Infectious Disease
Internal Medicine
Klebsiella - genetics
Klebsiella Infections - microbiology
Klebsiella pneumoniae - genetics
Klebsiella species
Microbial Sensitivity Tests - methods
Molecular identification
Multiplex Polymerase Chain Reaction - methods
bla LEN
bla SHV
molecular identification
class A β-lactamase
Klebsiella species
bla OKP
blaSHV
Class A β-lactamase
blaLEN
Molecular identification
blaOKP
bla(LEN)
bla(OKP)
bla(SHV)
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Summary:Abstract Klebsiella pneumoniae , Klebsiella variicola and Klebsiella quasipneumoniae are difficult to differentiate phenotypically, leading to misinterpretation of their infection prevalence. We propose a multiplex PCR for blaSHV, LEN and OKP and their flanking gene ( deo R). Since this scheme focuses only on chromosomal genes, it will be feasible for Klebsiella identification in the clinical routine.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0732-8893
1879-0070
DOI:10.1016/j.diagmicrobio.2017.01.005