Luteolin enhances oxidative stress tolerance via the daf-16 pathway in the nematode Caenorhabditis elegans

• Published in: Food Science and Technology Research Vol. 30; no. 2; pp. 253 - 260
• Main Authors: Yamamoto, Ryoko, Takahashi, Eriko, Harada, Maho, Sawahata, Izumi, Nishizuka, Makoto
• Format: Journal Article
• Language: English
• Published: Tsukuba Japanese Society for Food Science and Technology 01.01.2024; Japan Science and Technology Agency
• Subjects: Caenorhabditis elegans; Cytotoxicity; daf-16; Gene expression; Life span; lifespan; luteolin; Lymphocytes T; MnSOD gene; Oxidative stress; Prolongation; Sod; Worms
• ISSN: 1344-6606; 1881-3984
• DOI: 10.3136/fstr.FSTR-D-23-00176

Luteolin, widely present in plants, has various physiological functions. Here, we aimed to investigate whether luteolin affects lifespan and oxidative stress tolerance using Caenorhabditis elegans. Luteolin had no effect on the lifespan of wild-type N2 worms under normal conditions. In contrast, luteolin prolonged the lifespan of N2 worms under oxidative stress, suggesting that luteolin enhances oxidative stress tolerance. Luteolin upregulated the expression of gst-4, sod-1, sod-2, sod-3, and ctl-3 in N2 worms under oxidative stress conditions. The expression of these genes was regulated by DAF-16. Prolongation of lifespan and induction of gene expression by luteolin under oxidative stress conditions were not seen in daf-16 (mu86) mutants. These results suggest that the enhancement of oxidative stress tolerance by luteolin was mediated by the daf-16 pathway in C. elegans.