Development of a novel Sinapis arvensis disomic addition line in Brassica napus containing the restorer gene for Nsa CMS and improved resistance to Sclerotinia sclerotiorum and pod shattering

• Published in: Theoretical and applied genetics Vol. 120; no. 6; pp. 1089 - 1097
• Main Authors: Wei, Wenhui, Li, Yunchang, Wang, Lijun, Liu, Shengyi, Yan, Xiaohong, Mei, Desheng, Li, Yinde, Xu, Yusong, Peng, Pengfei, Hu, Qiong
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01.04.2010; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Agricultural production; Agriculture; Ascomycota - physiology; Biochemistry; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Botanical research; Brassica napus; Brassica napus - genetics; Brassica napus - immunology; Brassica napus - microbiology; Breeding; Canola; Cell differentiation, maturation, development, hematopoiesis; Cell physiology; Chromosomes, Plant - genetics; Classical genetics, quantitative genetics, hybrids; Crops; Cultivars; Cytoplasm; Diseases and pests; Fertility; Fundamental and applied biological sciences. Psychology; Fungi, Pathogenic; Gene expression; Genes; Genes, Plant - genetics; Genetic aspects; Genetic research; Genetics of eukaryotes. Biological and molecular evolution; Health aspects; Hybridization; Hybridization, Genetic; Immunity, Innate - genetics; Immunity, Innate - immunology; In Situ Hybridization; Life Sciences; Molecular and cellular biology; Original Paper; Plant Biochemistry; Plant Breeding/Biotechnology; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant Genetics and Genomics; Plant immunology; Plant Infertility - genetics; Plant Leaves - microbiology; Plant-pathogen relationships; Prevention; Pteridophyta, spermatophyta; Rape (Plant); Rape plants; Research centers; Sclerotinia sclerotiorum; Seeds; Selection, Genetic; Sinapis - genetics; Sinapis arvensis; Vegetals; China; Somatic Hybrid; Erucic Acid; Pollen Viability; Sterile Plant; Fertile Plant; Ascomycota; Addition line; Pod; Sclerotinia sclerotiorum; Fungi; Resistance; Gene; Cruciferae; Dicotyledones; Angiospermae; Brassica napus; Development; Genetics; Spermatophyta; Agriculture; Sinapis arvensis
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s00122-009-1236-6

An allo-cytoplasmic male sterile line, which was developed through somatic hybridization between Brassica napus and Sinapis arvensis (thus designated as Nsa CMS line), possesses high potential for hybrid production of rapeseed. In order to select for restorer lines, fertile plants derived from the same somatic hybridization combination were self-pollinated and testcrossed with the parental Nsa CMS line for six generations. A novel disomic alien addition line, B. napus-S. arvensis, has been successfully developed. GISH analysis showed that it contains one pair of chromosomes from S. arvensis and 19 pairs from B. napus, and retains stable and regular mitotic and meiotic processes. The addition line displays very strong restoration ability to Nsa CMS line, high resistance to Sclerotinia sclerotiorum and a low incidence of pod shattering. Because the addition line shares these very important agricultural characters, it is a valuable restorer to Nsa CMS line, and is named NR1 here (Nsa restorer no. 1).