Microbiological Sulfide Removal-From Microorganism Isolation to Treatment of Industrial Effluent

• Published in: Microorganisms (Basel) Vol. 9; no. 3; p. 611
• Main Authors: Yang, Zhendong, Liu, Zhenghua, Sklodowska, Aleksandra, Musialowski, Marcin, Bajda, Tomasz, Yin, Huaqun, Drewniak, Lukasz
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 16.03.2021; MDPI
• Subjects: Bacteria; Bacterial leaching; Cell culture; Chemical oxygen demand; Consortia; Cultivation; Effluents; Industrial effluents; Industrial wastewater; Leachates; Materials recovery; Metabolism; Mine wastes; Mining; Oxidation; Process controls; Reduction (metal working); Sulfate reduction; Sulfide; sulfide depletion; sulfide-oxidizing bacteria; Sulfides; Sulfur; sulfur production; Wastewater treatment; sulfide-oxidizing bacteria; sulfur production; sulfide depletion; sulfate reduction
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms9030611

Management of excessive aqueous sulfide is one of the most significant challenges of treating effluent after biological sulfate reduction for metal recovery from hydrometallurgical leachate. The main objective of this study was to characterize and verify the effectiveness of a sulfide-oxidizing bacterial (SOB) consortium isolated from post-mining wastes for sulfide removal from industrial leachate through elemental sulfur production. The isolated SOB has a complete sulfur-oxidizing metabolic system encoded by genes and is dominated by the genus. XRD analysis confirmed the presence of elemental sulfur in the collected sediment during cultivation of the SOB in synthetic medium under controlled physicochemical conditions. The growth yield after three days of cultivation reached ~2.34 g /mol , while approximately 84% of sulfide was transformed into elemental sulfur after 5 days of incubation. Verification of isolated SOB on the industrial effluent confirmed that it can be used for effective sulfide concentration reduction (~100% reduced from the initial 75.3 mg/L), but for complete leachate treatment (acceptable for discharged limits), bioaugmentation with other bacteria is required to ensure adequate reduction of chemical oxygen demand (COD).