CLIP170-like tip1p Spatially Organizes Microtubular Dynamics in Fission Yeast

• Published in: Cell Vol. 102; no. 5; pp. 695 - 704
• Main Authors: Brunner, Damian, Nurse, Paul
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.2000
• Subjects: Benzimidazoles - pharmacology; Carbamates; Carrier Proteins - chemistry; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell Polarity; CLIP170 protein; Cloning, Molecular; Fluorescent Antibody Technique; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Deletion; Genes, Fungal - genetics; Glycoproteins - chemistry; Glycoproteins - genetics; Glycoproteins - metabolism; Heat-Shock Proteins; Intermediate Filament Proteins; Kinetics; Microtubule-Associated Proteins - chemistry; Microtubules - drug effects; Microtubules - metabolism; Neoplasm Proteins; Paclitaxel - pharmacology; Phenotype; Protein Binding - drug effects; Schizosaccharomyces - cytology; Schizosaccharomyces - drug effects; Schizosaccharomyces - genetics; Schizosaccharomyces - metabolism; Schizosaccharomyces pombe; Schizosaccharomyces pombe Proteins; Sequence Homology, Amino Acid; tip1 protein
• ISSN: 0092-8674; 1097-4172
• DOI: 10.1016/S0092-8674(00)00091-X

Rod-shaped fission yeast cells grow in a polarized manner, and unlike budding yeast, the correct positioning of the growth sites at cell ends requires interphase microtubules. Here we describe a microtubule guidance mechanism that orients microtubules in the intracellular space along the long axis of the cell, guiding them to their target region at the cell ends. This mechanism involves tip1p, a CLIP170-like protein that localizes to distal tips of cytoplasmic microtubules. In the absence of tip1p, microtubular catastrophe is no longer restricted to cell ends but occurs when microtubules reach any region of the cellular cortex. Thus, tip1p enables microtubules to discriminate different cortical regions and regulates their dynamics accordingly.