ANTIBIOTIC BINDING TO HUMAN POLYMORPHONUCLEAR NEUTROPHILS, MOUSE LEUKEMIA L1210 CELLS AND MOUSE THYMOCYTES

• Published in: Journal of antibiotics Vol. 39; no. 1; pp. 141 - 148
• Main Authors: GRAY, GARY D., OHLMANN, GRETA M.
• Format: Journal Article
• Language: English
• Published: Tokyo JAPAN ANTIBIOTICS RESEARCH ASSOCIATION 1986; Japan Antibiotics Research Association
• Subjects: Animals; Anti-Bacterial Agents - metabolism; Antibacterial agents; Antibiotics. Antiinfectious agents. Antiparasitic agents; Biological and medical sciences; Clindamycin - metabolism; Doxycycline - metabolism; Fusidic Acid - metabolism; Humans; Leukemia L1210 - metabolism; Medical sciences; Mice; Neutrophils - metabolism; Novobiocin - metabolism; Phagocytes - metabolism; Pharmacology. Drug treatments; Rifampin - metabolism; T-Lymphocytes - metabolism; Tetracycline - metabolism; Thymidine - metabolism; Antibiotic; Fixation; Neutrophile; Thymocyte; Granulocyte; L1210-Leukemia; In vitro; Tumor cell; Activity metabolism relation
• ISSN: 0021-8820; 1881-1469
• DOI: 10.7164/antibiotics.39.141

This report describes a system in which antibiotics could be compared for binding to different mammalian cells. These included functional phagocytes (human polymorphonuclear neutrophils; PMNs), non-phagocytic lymphocytes (mouse thymocytes), and non-functional leukocytes (mouse leukemia L1210 cells). When antibiotics bound to PMNs, they bound about the same to L1210 cells but much less to thymocytes. Combining these data with previous data, the ranking of cells that bound the greatest amount of antibiotics was: PMNs=L1210 cells-blood mononuclear leukocytes>thymocytes>erythrocytes. Thus, antibiotics bind differentially and not indiscriminately to mammalian cells.