Temperature affects substrate-associated bacterial composition during Ganoderma lucidum hyphal growth

• Published in: Canadian journal of microbiology Vol. 67; no. 4; pp. 281 - 289
• Main Authors: Zhang, Bo, Zhou, Jie, Li, Xiaolin, Ye, Lei, Jia, Dinghong, Gan, Bingcheng, Tan, Wei
• Format: Journal Article
• Language: English
• Published: 1840 Woodward Drive, Suite 1, Ottawa, ON K2C 0P7 NRC Research Press 01.04.2021; Canadian Science Publishing NRC Research Press
• Subjects: Abundance; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; bacterial communities; bacterial composition; Bacterial growth; composition bactérienne; Culture Media; effet de la température; Environmental aspects; Firmicutes; Fungi; Fungiculture; Ganoderma lucidum; Growth; High temperature; high-throughput sequencing; Hot Temperature; hyphae; Hyphae - growth & development; Microbiota; Microorganisms; Morphology; Mushrooms; Mycelia; mycelium; Next-generation sequencing; Ochrobactrum; prediction; Reishi; Reishi - growth & development; Rhodococcus; Substrates; séquençage à haut débit; Temperature; temperature effect; China; composition bactérienne; Ganoderma lucidum; séquençage à haut débit; effet de la température; bacterial composition; high-throughput sequencing; temperature effect
• ISSN: 0008-4166; 1480-3275; 1480-3275
• DOI: 10.1139/cjm-2020-0070

The growth of the well-known fungus Ganoderma lucidum is influenced by temperature, which has an impact on the associated microbial structure in the substrate. In this study, we analyzed the bacterial diversity of the substrate at different temperatures using next-generation sequencing technology. A total of 513 733 sequences from 15 samples were assigned to 19 bacterial phyla. The samples were dominated by Proteobacteria, followed by Firmicutes; the 2 phyla exhibited opposite changes with elevated temperature. Bacterial genera showed different abundances at different temperatures, in which Sediminibacterium maintained a stable abundance below 40 °C, while Ochrobactrum and Rhodococcus were enriched with elevated temperature and both showed their highest abundances at 40 °C. Functional prediction uncovered 39 identified KEGG pathways, and bacterial genes involved in the membrane transport pathway exhibited the highest abundance subject to heat (40 °C) during the growth of G. lucidum. In general, our findings illustrated the influence of temperatures on G. lucidum mycelial morphology and the bacterial community in the substrate, and the results will facilitate cultivation of this fungus.