Modulation of the Expression of Components of the Stress Response by Dietary Arachidonic Acid in European Sea Bass (Dicentrarchus labrax) Larvae

• Published in: Lipids Vol. 50; no. 10; pp. 1029 - 1041
• Main Authors: Montero, Daniel, Terova, Genciana, Rimoldi, Simona, Betancor, Mónica B, Atalah, Eyad, Torrecillas, Silvia, Caballero, María J, Zamorano, María J, Izquierdo, Marisol
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.10.2015; Springer Nature B.V
• Subjects: Animals; antioxidants; Arachidonic acid; Arachidonic Acid - administration & dosage; Arachidonic Acid - pharmacology; Bass - genetics; Bass - growth & development; Biomedical and Life Sciences; catalase; cortisol; Dicentrarchus labrax; docosahexaenoic acid; eicosapentaenoic acid; experimental diets; fish; Fish Proteins - genetics; gene expression; gene expression regulation; Gene Expression Regulation - drug effects; genes; Glucocorticoid receptor; glucocorticoid receptors; glutathione peroxidase; heat shock proteins; HSP; Larva - drug effects; Larvae; Life Sciences; Lipidology; Medical Biochemistry; Medicinal Chemistry; messenger RNA; Microbial Genetics and Genomics; Neurochemistry; Nutrition; Original Article; Real-Time Polymerase Chain Reaction - veterinary; regulatory proteins; reverse transcriptase polymerase chain reaction; steroidogenesis; Stress; stress response; Stress, Physiological - drug effects; superoxide dismutase; HSP; Arachidonic acid; Stress; Glucocorticoid receptor; Dicentrarchus labrax
• ISSN: 0024-4201; 1558-9307
• DOI: 10.1007/s11745-015-4057-1

This study reports for the first time on European sea bass, Dicentrarchus labrax (L.), larvae, the effect of different levels of dietary arachidonic acid (ARA; 20:4n-6) on the expression of genes related to the fish stress response. Copies of mRNA from genes related to steroidogenesis [StAR (steroidogenic acute regulatory protein), c-Fos, and CYP11β (11β-hydroxylase gene)], glucocorticoid receptor complex [GR (glucocorticoid receptor) and HSP (heat shock proteins) 70 and 90) and antioxidative stress (catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase] were quantified. Eighteen day-old larvae were fed for 14 days with three experimental diets with increasing levels of ARA (0.3, 0.6 and 1.2 % d.w.) and similar levels of docosahexaenoic (22:6n-3) and eicosapentaenoic (20:5n-3) acids (5 and 3 %, respectively). The quantification of stress-related genes transcripts was conducted by One-Step TaqMan real time RT-PCR with the standard curve method (absolute quantification). Increase dietary levels of ARA induced a significantly (p < 0.05) down-regulation of genes related to cortisol synthesis, such as StAR and CYP11β and up-regulated genes related to glucocorticoid receptor complex, such as HSP70 and GR. No effects were observed on antioxidant enzymes gene expression. These results revealed the regulatory role of dietary ARA on the expression of stress-related genes in European sea bass larvae.