Purification, characterization, and gene cloning of cis, cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17

• Published in: FEMS microbiology letters Vol. 231; no. 1; pp. 119 - 124
• Main Authors: Murakami, Shuichiro, Kohsaka, Chihiro, Okuno, Takao, Takenaka, Shinji, Aoki, Kenji
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 09.02.2004; Blackwell Publishing Ltd; Blackwell
• Subjects: Amino Acid Sequence; Arthrobacter; Arthrobacter - enzymology; Arthrobacter - genetics; Arthrobacter - metabolism; Bacteriology; Benzamide; Benzamides - metabolism; Biological and medical sciences; Catechol metabolism; Catechols - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genes, Regulator; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Intramolecular Lyases - genetics; Intramolecular Lyases - isolation & purification; Intramolecular Lyases - metabolism; Microbiology; Miscellaneous; Molecular Sequence Data; Muconate cycloisomerase; Mycology; Phylogeny; Streptomyces setonii; β-Ketoadipate pathway; β-Ketoadipate pathway; Muconate cycloisomerase; CMC, chloro- cis, cis-muconate cycloisomerase; MC, cis, cis-muconate cycloisomerase; ORF, open reading frame; Catechol metabolism; Benzamide; Arthrobacter; CD, catechol 1,2-dioxygenase; Purification; Enzyme; Pyrocatechol; Metabolism; Isomerases; Gene; Intramolecular lyases; Bacteria; Actinomycetes

cis, cis-Muconate cycloisomerase (MC) was purified to homogeneity from benzamide-assimilating Arthrobacter sp. BA-5-17. The purified enzyme showed high activities for cis, cis-muconate and 3-methyl- cis, cis-muconate, and preferred the 3-substituted derivatives over the derivatives with the same sub...