Purification, characterization, and gene cloning of cis, cis-muconate cycloisomerase from benzamide-assimilating Arthrobacter sp. BA-5-17

• Published in: FEMS microbiology letters Vol. 231; no. 1; pp. 119 - 124
• Main Authors: Murakami, Shuichiro, Kohsaka, Chihiro, Okuno, Takao, Takenaka, Shinji, Aoki, Kenji
• Format: Journal Article
• Language: English
• Published: Oxford, UK Elsevier B.V 09.02.2004; Blackwell Publishing Ltd; Blackwell
• Subjects: Amino Acid Sequence; Arthrobacter; Arthrobacter - enzymology; Arthrobacter - genetics; Arthrobacter - metabolism; Bacteriology; Benzamide; Benzamides - metabolism; Biological and medical sciences; Catechol metabolism; Catechols - metabolism; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genes, Regulator; Growth, nutrition, metabolism, transports, enzymes. Molecular biology; Intramolecular Lyases - genetics; Intramolecular Lyases - isolation & purification; Intramolecular Lyases - metabolism; Microbiology; Miscellaneous; Molecular Sequence Data; Muconate cycloisomerase; Mycology; Phylogeny; Streptomyces setonii; β-Ketoadipate pathway; β-Ketoadipate pathway; Muconate cycloisomerase; CMC, chloro- cis, cis-muconate cycloisomerase; MC, cis, cis-muconate cycloisomerase; ORF, open reading frame; Catechol metabolism; Benzamide; Arthrobacter; CD, catechol 1,2-dioxygenase; Purification; Enzyme; Pyrocatechol; Metabolism; Isomerases; Gene; Intramolecular lyases; Bacteria; Actinomycetes
• ISSN: 0378-1097; 1574-6968
• DOI: 10.1016/S0378-1097(03)00933-9

cis, cis-Muconate cycloisomerase (MC) was purified to homogeneity from benzamide-assimilating Arthrobacter sp. BA-5-17. The purified enzyme showed high activities for cis, cis-muconate and 3-methyl- cis, cis-muconate, and preferred the 3-substituted derivatives over the derivatives with the same substituent at the 2 position as a substrate. A gene encoding MC of strain BA-5-17 was cloned and named catB. The catB gene was clustered with catR encoding a putative LysR-type regulator, catC encoding a putative muconolactone isomerase, and catA-II encoding the catechol 1,2-dioxygenase isozymes CD-III-1 and III-2. These genes showed the same orientation in transcriptional direction and the organization of cloned genes was catRBCA-II. In the phylogenetic analysis of MCs and chloro-MCs, the BA-5-17 and Streptomyces setonii MCs formed a subfamily, clearly distinguished from those of other MCs.