PRMT5 regulates ovarian follicle development by facilitating Wt1 translation
Protein arginine methyltransferase 5 ( ) is the major type II enzyme responsible for symmetric dimethylation of arginine. Here, we found that PRMT5 was expressed at high level in ovarian granulosa cells of growing follicles. Inactivation of in granulosa cells resulted in aberrant follicle developmen...
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Published in | eLife Vol. 10 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
England
eLife Science Publications, Ltd
27.08.2021
eLife Sciences Publications Ltd eLife Sciences Publications, Ltd |
Subjects | |
Online Access | Get full text |
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Summary: | Protein arginine methyltransferase 5 (
) is the major type II enzyme responsible for symmetric dimethylation of arginine. Here, we found that PRMT5 was expressed at high level in ovarian granulosa cells of growing follicles. Inactivation of
in granulosa cells resulted in aberrant follicle development and female infertility. In
knockout mice, follicle development was arrested with disorganized granulosa cells in which WT1 expression was dramatically reduced and the expression of steroidogenesis-related genes was significantly increased. The premature differentiated granulosa cells were detached from oocytes and follicle structure was disrupted. Mechanism studies revealed that
expression was regulated by PRMT5 at the protein level. PRMT5 facilitated IRES-dependent translation of
mRNA by methylating HnRNPA1. Moreover, the upregulation of steroidogenic genes in
-deficient granulosa cells was repressed by
overexpression. These results demonstrate that PRMT5 participates in granulosa cell lineage maintenance by inducing
expression. Our study uncovers a new role of post-translational arginine methylation in granulosa cell differentiation and follicle development. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work. Two of the authors have the name 'Min Chen'. |
ISSN: | 2050-084X 2050-084X |
DOI: | 10.7554/elife.68930 |