Effects of Storage Temperature on the Effective Activity of Straw Frozen Semen after Thawing

• Published in: Animal husbandry and feed science Vol. 7; no. 4; pp. 203 - 204
• Main Authors: Limin, Qiao, Jianmin, Ma, Fuqiang, Qiao, Hua, Yao, Xishan, Xiao, Yinxu, Hou, Jingtao, Fu, Kang, Ren
• Format: Journal Article
• Language: English
• Published: Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.08.2015
• Subjects: 人工授精; 冷冻精液; 授精时间; 活性; 精子活力; 精子运动能力; 精液解冻; 贮藏温度
• ISSN: 1943-9911

The aim was to discuss the optimal storage environment and proper insemination time after thawing of 0.25 mL straw frozen semen. Straw frozen semen was thawed at 40 ℃ for 20 s, and then stored at 0 -4 ℃, 14 - 16 ℃, 25 -27 ℃ for 2, 4, 6, 8 and 10 h, respectively. The sperm motility was detected. After thawing, semen was stored at 0 - 4 ℃ and 14 - 16 ℃ for 10 h. Their sperm motilities （0.434 ±0. 016 7 and 0.423 ±0.019 6） had no significant differences （P 〉 0.05） with initial thawing motility （0.441 ± 0.030）. Sperm motility reduced as the storage time prolonged at 25 -27 ℃. Sperm motility after 6 h had signifi- cant differences with that of initial thawing motility （P 〈 O. 05 ）, and sperm motilities after 8 and 10 h showed extremely significant differences （P 〈 0.01 ）. Thus, sperm motility after thawing was still very high after stored at 0 -4 ℃ and 14 - 16 ℃ within 10 h, which met the requirements for insemination. Under this temperature and time ranges, sperm could be carried over long distances, which had small effects on sperm quality and reached the expected insemination effects. However, under the temperature of 25 - 27 ℃, semen should be used for insemination within 6 h after thawing.