Effects of divalent cations on Halobacterium salinarum cell aggregation

• Published in: Journal of bioscience and bioengineering Vol. 104; no. 1; pp. 42 - 46
• Main Authors: Kawakami, Yoshitaka, Hayashi, Nobuhiro, Ema, Mizue, Nakayama, Masashi
• Format: Journal Article
• Language: English
• Published: Amsterdarm Elsevier B.V 01.07.2007; Elsevier Science; Elsevier Limited
• Subjects: AGUA SALINA; BESOIN NUTRITIONNEL; Biological and medical sciences; BIOREACTEUR; BIOREACTORS; BIORREACTORES; Biotechnology; Ca 2+-dependent cell aggregation; Calcium - pharmacology; CATION; CATIONES; CATIONS; Cations, Divalent - pharmacology; divalent cation; EAU SALINE; Fundamental and applied biological sciences. Psychology; Halobacterium salinarum; Halobacterium salinarum - drug effects; halophilic Archaea; Metals - pharmacology; NECESIDADES DE NUTRIENTES; NUTRITIONAL REQUIREMENTS; PROCARIOTICA; PROKARYOTES; PROTOCARYOTE; SALINE WATER; SALT TOLERANCE; TOLERANCE AU SEL; TOLERANCIA A LA SAL; divalent cation; Ca 2+-dependent cell aggregation; Halobacterium salinarum; halophilic Archaea; Aggregation; Calcium; Archaeobacteria; Halophily; Halobacteriales; Ca2+-dependent cell aggregation; Bacteria; Cations; Halobacteriaceae
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1263/jbb.104.42

Ca 2+ was found to be essential for initiating Halobacterium salinarum CCM 2090 cell aggregation. The floc formed from such aggregation could easily be dissociated without cellular lysis by sodium citrate. Cr 2+, Mn 2+, Fe 3+, Co 2+, Ni 2+, Cu 2+, and Zn 2+ could replace Ca 2+. However, Mg 2+, Sr 2+, Mo 2+, Cd 2+, Sn 2+, Hg 2+, and Pb 2+ induced no flocculation of cells of this halophilic archaeon. Mg 2+ acted antagonistically against Ca 2+-induced aggregation. Such aggregation might be directly caused by the interaction of Ca 2+ and aggregation factors from 55°C-treated cell extract.