Chicken hemogen homolog is involved in the chicken-specific sex-determining mechanism
Using a comprehensive transcriptome analysis, a Z chromosome-linked chicken homolog of hemogen (cHEMGN) was identified and shown to be specifically involved in testis differentiation in early chicken embryos. Hemogen [ Hemgn in mice, EDAG (erythroid differentiation-associated gene protein) in humans...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 110; no. 9; pp. 3417 - 3422 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
26.02.2013
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | Using a comprehensive transcriptome analysis, a Z chromosome-linked chicken homolog of hemogen (cHEMGN) was identified and shown to be specifically involved in testis differentiation in early chicken embryos. Hemogen [ Hemgn in mice, EDAG (erythroid differentiation-associated gene protein) in humans] was recently characterized as a hematopoietic tissue-specific gene encoding a transcription factor that regulates the proliferation and differentiation of hematopoietic cells in mammals. In chicken, cHEMGN was expressed not only in hematopoietic tissues but also in the early embryonic gonad of male chickens. The male-specific expression was identified in the nucleus of (pre)Sertoli cells after the sex determination period and before the expression of SOX9 (SRY-box 9). The expression of cHEMGN was induced in ZW embryonic gonads that were masculinized by aromatase inhibitor treatment. ZW embryos overexpressing cHEMGN , generated by infection with retrovirus carrying cHEMGN , showed masculinized gonads. These findings suggest that cHEMGN is a transcription factor specifically involved in chicken sex determination. |
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Bibliography: | http://dx.doi.org/10.1073/pnas.1218714110 Edited by Patricia K. Donahoe, Massachusetts General Hospital, Boston, MA, and approved January 18, 2013 (received for review October 29, 2012) Author contributions: A.K. designed research; T.N., M.I., N.A., and A.K. performed research; H.I. contributed new reagents/analytic tools; T.N., M.I., N.A., H.I., and A.K. analyzed data; and T.N. and A.K. wrote the paper. |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.1218714110 |