Daphnia magna and Xenopus laevis as in vivo models to probe toxicity and uptake of quantum dots functionalized with gH625

• Published in: International journal of nanomedicine Vol. 12; pp. 2717 - 2731
• Main Authors: Galdiero, Emilia, Falanga, Annarita, Siciliano, Antonietta, Maselli, Valeria, Guida, Marco, Carotenuto, Rosa, Tussellino, Margherita, Lombardi, Lucia, Benvenuto, Giovanna, Galdiero, Stefania
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2017; Dove Medical Press
• Subjects: Animal research models; Animals; Causes of; Cell penetrating peptide; Comparative analysis; Daphnia - drug effects; daphnia magna; delivery; Embryo, Nonmammalian - drug effects; Investigations; Mutagenicity Tests - methods; Nanoparticles - toxicity; Original Research; Peptides - chemistry; Peptides - toxicity; Physiological aspects; Properties; Quantum dots; Quantum Dots - chemistry; Quantum Dots - toxicity; Tissue Distribution; Toxicity; Toxicity Tests - methods; Viral Envelope Proteins - chemistry; Viral Envelope Proteins - toxicity; xenopus laevis; Xenopus laevis - embryology; Italy; genotoxicity; delivery; blood–brain barrier; nanoparticles; membranotropic peptide
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S127226

The use of quantum dots (QDs) for nanomedicine is hampered by their potential toxicologic effects and difficulties with delivery into the cell interior. We accomplished an in vivo study exploiting and to evaluate both toxicity and uptake of QDs coated with the membranotropic peptide gH625 derived from the glycoprotein H of herpes simplex virus and widely used for drug delivery studies. We evaluated and compared the effects of QDs and gH625-QDs on the survival, uptake, induction of several responsive pathways and genotoxicity in , and we found that QDs coating plays a key role. Moreover, studies on embryos allowed to better understand their cell/tissue localization and delivery efficacy. embryos raised in Frog Embryo Teratogenesis Assay- containing QDs or gH625-QDs showed that both nanoparticles localized in the gills, lung and intestine, but they showed different distributions, indicating that the uptake of gH625-QDs was enhanced; the functionalized QDs had a significantly lower toxic effect on embryos' survival and phenotypes. We observed that and are useful in vivo models for toxicity and drug delivery studies.