Using a functional enzyme model to understand the chemistry behind hydrogen sulfide induced hibernation
The toxic gas H₂S is produced by enzymes in the body. At moderate concentrations, H₂S elicits physiological effects similar to hibernation. Herein, we describe experiments that imply that the phenomenon probably results from reversible inhibition of the enzyme cytochrome c oxidase (CcO), which reduc...
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Published in | Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 52; pp. 22090 - 22095 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
United States
National Academy of Sciences
29.12.2009
National Acad Sciences |
Subjects | |
Online Access | Get full text |
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Summary: | The toxic gas H₂S is produced by enzymes in the body. At moderate concentrations, H₂S elicits physiological effects similar to hibernation. Herein, we describe experiments that imply that the phenomenon probably results from reversible inhibition of the enzyme cytochrome c oxidase (CcO), which reduces oxygen during respiration. A functional model of the oxygen-reducing site in CcO was used to explore the effects of H₂S during respiration. Spectroscopic analyses showed that the model binds two molecules of H2S. The electro-catalytic reduction of oxygen is reversibly inhibited by H₂S concentrations similar to those that induce hibernation. This phenomenon derives from a weak, reversible binding of H₂S to the FeII porphyrin, which mimics heme a₃ in CcO's active site. No inhibition of CcO is detected at lower H₂S concentrations. Nevertheless, at lower concentrations, H₂S could have other biological effects on CcO. For example, H₂S rapidly reduces FeIII and CuII in both the oxidized form of this functional model and in CcO itself. H₂S also reduces CcO's biological reductant, cytochrome c, which normally derives its reducing equivalents from food metabolism. Consequently, it is speculated that H₂S might also serve as a source of electrons during periods of hibernation when food supplies are low. |
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Bibliography: | Author Contributions: J.P.C., S.G., A.D., and R.A.D. designed research; S.G., A.D., and R.A.D. performed research; S.G., A.D., and R.A.D. analyzed data; and J.P.C., S.G., and R.A.D. wrote the paper. Contributed by James P. Collman, April 13, 2009 |
ISSN: | 0027-8424 1091-6490 |
DOI: | 10.1073/pnas.0904082106 |