miR-382 inhibits migration and invasion by targeting ROR1 through regulating EMT in ovarian cancer

• Published in: International journal of oncology Vol. 48; no. 1; pp. 181 - 190
• Main Authors: TAN, HONG, HE, QINGNAN, GONG, GUANHUI, WANG, YIXUAN, LI, JUANNI, WANG, JUNPU, ZHU, DING, WU, XIAOYING
• Format: Journal Article
• Language: English
• Published: Greece D.A. Spandidos 01.01.2016; Spandidos Publications; Spandidos Publications UK Ltd
• Subjects: Carcinogenesis - genetics; Cell Line, Tumor; Cell Movement - genetics; Cell Proliferation - genetics; Cell receptors; Development and progression; epithelial-mesenchymal transition; Epithelial-Mesenchymal Transition - genetics; Female; Gene expression; Gene Expression Regulation, Neoplastic; Genetic aspects; Health aspects; Humans; invasion; Medical prognosis; Metastasis; MicroRNA; MicroRNAs; MicroRNAs - biosynthesis; MicroRNAs - genetics; migration; miR-382; Neoplasm Invasiveness - genetics; Ovarian cancer; Ovarian Neoplasms - genetics; Ovarian Neoplasms - pathology; Prognosis; Proteins; receptor tyrosine kinase orphan receptor 1; Receptor Tyrosine Kinase-like Orphan Receptors - biosynthesis; Receptor Tyrosine Kinase-like Orphan Receptors - genetics; United States > US; China
• ISSN: 1019-6439; 1791-2423
• DOI: 10.3892/ijo.2015.3241

Increasing evidence suggests that microRNAs (miRNAs) play a critical role in tumorigenesis. Decreased expression of miR-382 has been observed in various types of cancers. However, the biological function of miRNA-382 in ovarian cancer is still largely unknown. Here, we found miR-382 was downregulated in human ovarian cancer tissues and cell lines. miR-382 inhibited ovarian cancer cell proliferation, migration, invasion and the epithelial-mesenchymal transition (EMT). Furthermore, we identified receptor tyrosine kinase orphan receptor 1 (ROR1) as a target of miR-382, and miR-382 rescued the promotion effect of ROR1 on migration, invasion and EMT process in SKOV3 and COV434 cells. Collectively, these findings revealed that miR-382 inhibits migration and invision by targeting ROR1 through regulating EMT in ovarian cancer, and might serve as a tumor suppressor in ovarian cancer.