Endogenous Apelin Is Protective Against Age-Associated Loss of Retinal Ganglion Cells in Mice

• Published in: Frontiers in aging neuroscience Vol. 12; p. 58
• Main Authors: Ishimaru, Yuki, Sumino, Akihide, Shibagaki, Fumiya, Yamamuro, Akiko, Yoshioka, Yasuhiro, Maeda, Sadaaki
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Research Foundation 20.03.2020; Frontiers Media S.A
• Subjects: Age; Aging; apelin; apelin receptor (APJ); cell loss; Degeneration; Electrodes; Gene expression; Immunofluorescence; knock-out; Neuroscience; Pharmaceutical industry; Polymerase chain reaction; Retina; retinal ganglion cell (RGC); Retinal ganglion cells; Rodents; United States > US; Japan; aging; retinal ganglion cell (RGC); apelin; knock-out; apelin receptor (APJ); cell loss
• ISSN: 1663-4365; 1663-4365
• DOI: 10.3389/fnagi.2020.00058

Age-associated loss of retinal ganglion cells (RGCs) causes visual deficits, but there is not yet any therapeutic agent to prevent the loss of these cells. Herein, we report that apelin, an endogenous peptide ligand of APJ receptor, is protective against the age-related loss of RGCs in mice. The mRNA expression of apelin was reduced in the retina of old mice compared with that in young mice, whereas retinal APJ expression increased with age. Immunofluorescence staining showed that APJ was present in RGCs and their surrounding cells expressed apelin. In addition, both functional and histological analyses demonstrated that apelin deficiency accelerated the loss of RGCs associated with age in mice. These results suggest that endogenous apelin plays a protective role against the degeneration of RGCs and that the apelinergic axis may be a new target for preventing age-related visual impairment.