Hybrid Cell Membrane-Functionalized Biomimetic Nanoparticles for Targeted Therapy of Osteosarcoma

• Published in: International journal of nanomedicine Vol. 17; pp. 837 - 854
• Main Authors: Cai, Jia-Xin, Liu, Ji-Hua, Wu, Jun-Yong, Li, Yong-Jiang, Qiu, Xiao-Han, Xu, Wen-Jie, Xu, Ping, Xiang, Da-Xiong
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01.01.2022; Dove; Dove Medical Press
• Subjects: Animals; B cells; biomimetic nano-drug delivery system; Biomimetics; Bone Neoplasms - drug therapy; Cancer; Cell Line, Tumor; Cell Membrane; Chemotherapy; Drug Carriers - chemistry; Drug delivery systems; Drugs; Health aspects; High performance liquid chromatography; Humans; Lactic Acid - chemistry; Mice; Nanoparticles; Nanoparticles - chemistry; Original Research; Osteosarcoma; Osteosarcoma - drug therapy; Paclitaxel; Polyglycolic Acid - chemistry; Polylactic Acid-Polyglycolic Acid Copolymer; Scientific equipment and supplies industry; targeted therapy; Tissue Distribution; Vehicles; United States; China; paclitaxel; targeted therapy; biomimetic nano-drug delivery system; osteosarcoma
• ISSN: 1178-2013; 1176-9114; 1178-2013
• DOI: 10.2147/IJN.S346685

In order to prepare a biomimetic nano-carrier which has inflammatory chemotaxis, homologous targeting and reduce immune clearance, for targeted chemotherapy of osteosarcoma, we fabricated the paclitaxel-loaded poly(lactic-co-glycolic) acid (PLGA) nanoparticles coated with 143B-RAW hybrid membrane (PTX-PLGA@[143B-RAW] NPs) and evaluate its anti-cancer efficacy in vitro and vivo. PTX-PLGA@[143B-RAW] NPs were prepared by the ultrasonic method and were characterized by size, zeta potential, polymer dispersion index (PDI), Coomassie bright blue staining, transmission electron microscopy (TEM) and high performance liquid chromatography (HPLC). Cellular uptake, cell viability assay, flow cytometry and chemotactic effect of PTX-PLGA@[143B-RAW] NPs were evaluated in vitro. Biodistribution, anti-cancer therapeutic efficacy and safety of PTX-PLGA@[143B-RAW] NPs were evaluated in 143B osteosarcoma xenograft mice. The hybrid membrane successfully coated onto the surface of PLGA nanoparticles. PTX-PLGA@[143B-RAW] NPs had a drug loading capacity of 4.24 ± 0.02% and showed targeting ability to osteosarcoma. PTX-PLGA@[143B-RAW] NPs showed high cellular uptake and improved anti-cancer efficacy against 143B cells. More importantly, PTX-PLGA@[143B-RAW] NPs treatment suppressed tumor growth in tumor-bearing mice with minimal damage to normal tissues. PTX-PLGA@[143B-RAW] NPs could be used for targeted drug delivery and osteosarcoma therapy.