Matrix metalloproteinase-9 activity in human lens epithelial cells of cortical, posterior subcapsular, and nuclear cataracts

• Published in: Journal of cataract and refractive surgery Vol. 34; no. 12; pp. 2063 - 2067
• Main Authors: Alapure, Bhagwat V., Praveen, Mamidipudi R., Gajjar, Devarshi, Vasavada, Abhay R., Rajkumar, Sankaranarayanan, Johar, Kaid
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.12.2008; Elsevier
• Subjects: Biological and medical sciences; Cataract - enzymology; Epithelial Cells - enzymology; Humans; Lens Capsule, Crystalline - enzymology; Lens Capsule, Crystalline - pathology; Lens Cortex, Crystalline - enzymology; Lens Cortex, Crystalline - pathology; Lens diseases; Lens Nucleus, Crystalline - enzymology; Lens Nucleus, Crystalline - pathology; Lens, Crystalline - enzymology; Matrix Metalloproteinase 9 - metabolism; Medical sciences; Middle Aged; Ophthalmology; Phacoemulsification; Prospective Studies; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Surgery of the eye and orbit; Human; Cataract; Enzyme; Metalloendopeptidases; Activity; Subcapsular; Gelatinase B; Peptidases; Eye disease; Lens disease; Treatment; Surgery; Posterior; Hydrolases; Epithelial cell; Lens; Ophthalmology; Anterior segment disease
• ISSN: 0886-3350; 1873-4502
• DOI: 10.1016/j.jcrs.2008.08.016

To evaluate the level of matrix metalloproteinase-9 (MMP-9) activity in lens epithelial cells (LECs) derived from different types of cataract in patients having phacoemulsification. Iladevi Cataract & IOL Research Centre, Memnagar, Ahmedabad, India. This observational study of 275 patients having phacoemulsification was performed to evaluate the level of MMP-9 activity in LECs. All anterior lens capsules harboring the LECs and derived from the surgical curvilinear capsulorhexis were obtained during phacoemulsification. The anterior lens capsule samples were processed to analyze MMP-9 activity using a succinylated gelatin assay. The samples were grouped based on age and on pure cataract type. The level of MMP-9 activity in LECs was highest in eyes with cortical cataract. A significant difference in the level of MMP-9 activity was found in different types of cataract ( P<.001). The highest level of MMP-9 activity was in patients older than 60 years. The MMP-9 activity increased gradually with age irrespective of cataract type ( P<.001). A significant difference was observed in the level of MMP-9 activity between different types of cataract. The activity of MMP-9 was highest in eyes with cortical cataract. The level of MMP-9 activity increased with age in the LECs of patients with age-related cataract.