Transcriptome Analysis of Induced Systemic Drought Tolerance Elicited by Pseudomonas chlororaphis O6 in Arabidopsis thaliana

• Published in: The plant pathology journal Vol. 29; no. 2; pp. 209 - 220
• Main Authors: Cho, S.M., Chunnam Techno University, Jeonnam, Republic of Korea, Kang, B.R., Jeollanamdo Agricultural Research and Extension Services, Naju , Republic of Korea, Kim, Y.C., Chonnam National University, Gwangju, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Korea (South) 한국식물병리학회 01.06.2013; Korean Society of Plant Pathology; Hanrimwon Publishing Company
• Subjects: ENFERMEDADES DE LAS PLANTAS; induced systemic tolerance; induced systemic tolerance,plant disease resistance,transcript analysis; MALADIE DES PLANTES; plant disease resistance; PLANT DISEASES; plant growth-promoting rhizobacteria; RHIZOBACTERIA; RHIZOBACTERIE; RIZOBACTERIAS; transcript analysis; 농학; plant disease resistance; transcript analysis; induced systemic tolerance; plant growth-promoting rhizobacteria
• ISSN: 1598-2254; 2093-9280
• DOI: 10.5423/PPJ.SI.07.2012.0103

Root colonization by Pseudomonas chlororaphis O6 induces systemic drought tolerance in Arabidopsis thaliana. Microarray analysis was performed using the 22,800- gene Affymetrix GeneChips to identify differentiallyexpressed genes from plants colonized with or without P. chlororaphis O6 under drought stressed conditions or normal growth conditions. Root colonization in plants grown under regular irrigation condition increased transcript accumulation from genes associated with defense, response to reactive oxygen species, and auxin- and jasmonic acid-responsive genes, but decreased transcription factors associated with ethylene and abscisic acid signaling. The cluster of genes involved in plant disease resistance were up-regulated, but the set of drought signaling response genes were down-regulated in the P. chlororaphis O6-colonized under drought stress plants compared to those of the drought stressed plants without bacterial treatment. Transcripts of the jasmonic acid-marker genes, VSP1 and pdf-1.2, the salicylic acid regulated gene, PR-1, and the ethylene-response gene, HEL, also were up-regulated in plants colonized by P. chlororaphis O6, but differed in their responsiveness to drought stress. These data show how gene expression in plants lacking adequate water can be remarkably influenced by microbial colonization leading to plant protection, and the activation of the plant defense signal pathway induced by root colonization of P. chlororaphis O6 might be a key element for induced systemic tolerance by microbes.