Streptococcus canis Are a Single Population Infecting Multiple Animal Hosts Despite the Diversity of the Universally Present M-Like Protein SCM

• Published in: Frontiers in microbiology Vol. 10; p. 631
• Main Authors: Pinho, Marcos D, Foster, Geoffrey, Pomba, Constança, Machado, Miguel P, Baily, Johanna L, Kuiken, Thijs, Melo-Cristino, José, Ramirez, Mário
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 29.03.2019
• Subjects: genome; M-like protein (SCM) gene; Microbiology; multilocus sequence typing; Streptococcus canis; wildlife; Streptococcus canis; M-like protein (SCM) gene; genome; multilocus sequence typing; wildlife
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2019.00631

is an animal pathogen which occasionally causes infections in humans. The M-like protein (SCM) encoded by the gene, is its best characterized virulence factor but previous studies suggested it could be absent in a substantial fraction of isolates. We studied the distribution and variability of the gene in 188 isolates recovered from companion animals ( = 152), wild animal species ( = 20), and humans ( = 14). Multilocus sequence typing, including the first characterization of wildlife isolates, showed that the same lineages are present in all animal hosts, raising the possibility of extensive circulation between species. Whole-genome analysis revealed that -like genes found previously in correspond to divergent genes, indicating that what was previously believed to correspond to two genes is in fact the same locus. We designed primers allowing for the first time the successful amplification of the gene in all isolates. Analysis of the sequences identified 12 distinct types, which could be divided into two clusters: group I (76%, = 142) and group II (24%, = 46) sharing little sequence similarity. The predicted group I SCM showed extensive similarity with each other outside of the -terminal hypervariable region and a conserved IgG binding domain. This domain was absent from group II SCM variants found in isolates previously thought to lack the gene, which also showed greater amino acid variability. Further studies are necessary to elucidate the possible host interacting partners of the group II SCM variants and their role in virulence.