Transfer RNA‐mediated suppression of amber stop codons in transgenic Arabidopsis thaliana
Summary An artificial amber suppressor tRNALeu gene (supL.) was physically linked to a mutated gus reporter gene, p35S‐gus(amL), which was inactivated by an amber stop codon (amL). Upon introduction into Arabidopsis thaliana, the presence of the supL. gene was found to be correlated with cytotoxic e...
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Published in | The Plant journal : for cell and molecular biology Vol. 11; no. 3; pp. 587 - 595 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Blackwell Science Ltd, Osney Mead, Oxford OX2 0EL, UK
Blackwell Science Ltd
01.03.1997
Blackwell Science |
Subjects | |
Online Access | Get full text |
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Summary: | Summary
An artificial amber suppressor tRNALeu gene (supL.) was physically linked to a mutated gus reporter gene, p35S‐gus(amL), which was inactivated by an amber stop codon (amL). Upon introduction into Arabidopsis thaliana, the presence of the supL. gene was found to be correlated with cytotoxic effects observed during tissue culture and in mature plants. Those primary transformants that displayed cytotoxic symptoms were shown by X‐Gluc staining to express GUS as a result of amber stop codon suppression in vivo. Phenotypically normal lines were found by RT‐PCR to express supL. GUS activity above background level was barely detectable in these plants, indicating a low level expression of supL. However, the remaining suppressor activity was still sufficient to transactivate an amber‐mutated male sterility gene, pA9‐barnase(amL1) when combined within the same plant by crossing. The suppressor tRNALeu gene may thus be used in transgenic plants for gene transactivation. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0960-7412 1365-313X |
DOI: | 10.1046/j.1365-313X.1997.11030587.x |