Synthesis, characterisation and bactericidal effect of ZnO nanoparticles via chemical and bio-assisted (Silybum marianum in vitro plantlets and callus extract) methods: a comparative study

• Published in: IET nanobiotechnology Vol. 12; no. 5; pp. 604 - 608
• Main Authors: Nazir, Saher, Zaka, Mehreen, Adil, Muhammad, Abbasi, Bilal Haider, Hano, Christophe
• Format: Journal Article
• Language: English
• Published: United States The Institution of Engineering and Technology 01.08.2018; Institution of Engineering and Technology (IET)
• Subjects: Anti-Bacterial Agents - chemical synthesis; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; antibacterial activity; Bacillus subtilis; Bacteria - drug effects; bio‐assisted green assembly; bio‐assisted methods; chemical methods; energy dispersive X‐ray analysis; Fourier transform infrared spectra; Fourier transform infrared spectroscopy; green chemistry; Green Chemistry Technology; II‐VI semiconductors; Klebsiella pneumonia; Life Sciences; Metal Nanoparticles - chemistry; nanobiotechnology; nanofabrication; nanoparticles; Nanotechnology - methods; Plant Extracts - chemistry; Plant Extracts - metabolism; Research Article; scanning electron microscopy; semiconductor growth; Silybum marianum - chemistry; Silybum marianum - cytology; Silybum marianum - metabolism; Silybum marianum in vitro callus extract methods; Silybum marianum in vitro plantlets methods; sodium hydroxide; Vegetal Biology; wide band gap semiconductors; X‐ray chemical analysis; X‐ray diffraction; zinc acetate; zinc compounds; Zinc Oxide - chemistry; Zinc Oxide - pharmacology; zinc oxide nanoparticles; ZnO; antibacterial activity; Fourier transform infrared spectra; Bacillus subtilis; nanofabrication; X-ray diffraction; green chemistry; semiconductor growth; nanoparticles; chemical methods; Silybum marianum in vitro plantlets methods; zinc compounds; bio-assisted methods; bio-assisted green assembly; scanning electron microscopy; Silybum marianum in vitro callus extract methods; nanobiotechnology; sodium hydroxide; energy dispersive X-ray analysis; Klebsiella pneumonia; II-VI semiconductors; zinc oxide nanoparticles; zinc acetate; X-ray chemical analysis; Fourier transform infrared spectroscopy; ZnO; wide band gap semiconductors; canning electron microscopy
• ISSN: 1751-8741; 1751-875X; 1751-875X
• DOI: 10.1049/iet-nbt.2017.0067

Currently, the evolution of green chemistry in the synthesis of nanoparticles (NPs) with the usage of plants has captivated a great response. In this study, in vitro plantlets and callus of Silybum marianum were exploited as a stabilising agent for the synthesis of zinc oxide (ZnO) NPs using zinc acetate and sodium hydroxide as a substitute for chemical method. The contemporary investigation defines the synthesis of ZnO NPs prepared by chemical and bio-extract-assisted methods. Characterisation techniques such as X-ray diffraction, scanning electron microscopy, Fourier transform infrared spectroscopy and energy dispersive X-ray were used to confirm the synthesis. Although chemical and bio-assisted methods are suitable choices for NPs synthesis, the bio-assisted green assembly is advantageous due to superior stability. Moreover, this report describes the antibacterial activity of the synthesised NPs against standard strains of Klebsiella pneumonia and Bacillus subtilis.